ArticleBMC microbiology2011
The streptococcal collagen-like protein-1 (Scl1) is a significant determinant for biofilm formation by group A Streptococcus.
Article in BMC microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 51 citations in OpenAlex.
- Recurrence following invasive GAS infections in adults: Triumph of virulence or failure of immunity?Virulence · 2025Article
- Nosocomial Transmission of Necrotizing Fasciitis: A Molecular Characterization of Group A Streptococcal DNases in Clinical Virulence.Microorganisms · 2024Article
- Prokaryotic Collagen-Like Proteins as Novel Biomaterials.Frontiers in bioengineering and biotechnology · 2022Review
- Consistent Biofilm Formation byFrontiers in microbiology · 2022Article
- Immune Cell Modulation of the Extracellular Matrix Contributes to the Pathogenesis of Pancreatic Cancer.Biomolecules · 2021Review
- Deciphering Streptococcal Biofilms.Microorganisms · 2020Review
- Phosphotransferase System Uptake and Metabolism of the β-Glucoside Salicin Impact Group A Streptococcal Bloodstream Survival and Soft Tissue Infection.Infection and immunity · 2020Article
- Singularities of Pyogenic Streptococcal Biofilms - From Formation to Health Implication.Frontiers in microbiology · 2020Review
- Streptococcus pyogenes Capsule Promotes Microcolony-Independent Biofilm Formation.Journal of bacteriology · 2019Article
- Adaptation of the group A Streptococcus adhesin Scl1 to bind fibronectin type III repeats within wound-associated extracellular matrix: implications for cancer therapy.Molecular microbiology · 2019Article
- Current Understanding of Group A Streptococcal Biofilms.Current drug targets · 2019Review
- Polymorphisms of a Collagen-Like Adhesin Contributes toFrontiers in microbiology · 2019Article
- Collagen-like proteins of pathogenic streptococci.Molecular microbiology · 2017Review
- YvqE and CovRS of Group A Streptococcus Play a Pivotal Role in Viability and Phenotypic Adaptations to Multiple Environmental Stresses.PloS one · 2017Article
- Metatranscriptomic insights on gene expression and regulatory controls in Candidatus Accumulibacter phosphatis.The ISME journal · 2016Article
- Unique Footprint in the scl1.3 Locus Affects Adhesion and Biofilm Formation of the Invasive M3-Type Group A Streptococcus.Frontiers in cellular and infection microbiology · 2016Article
- Article
- A direct, ratiometric, and quantitative MALDI-MS assay for protein methyltransferases and acetyltransferases.Analytical biochemistry · 2015Article
- Natural variant of collagen-like protein a in serotype M3 group a Streptococcus increases adherence and decreases invasive potential.Infection and immunity · 2015Article
- Streptococcus pyogenes biofilms-formation, biology, and clinical relevance.Frontiers in cellular and infection microbiology · 2015Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundGroup A Streptococcus (GAS) is a human-specific pathogen responsible for a number of diseases characterized by a wide range of clinical manifestations. During host colonization GAS-cell aggregates or microcolonies are observed in tissues. GAS biofilm, which is an in vitro equivalent of tissue microcolony, has only recently been studied and little is known about the specific surface determinants that aid biofilm formation. In this study, we demonstrate that surface-associated streptococcal collagen-like protein-1 (Scl1) plays an important role in GAS biofilm formation.
resultsBiofilm formation by M1-, M3-, M28-, and M41-type GAS strains, representing an intraspecies breadth, were analyzed spectrophotometrically following crystal violet staining, and characterized using confocal and field emission scanning electron microscopy. The M41-type strain formed the most robust biofilm under static conditions, followed by M28- and M1-type strains, while the M3-type strains analyzed here did not form biofilm under the same experimental conditions. Differences in architecture and cell-surface morphology were observed in biofilms formed by the M1- and M41-wild-type strains, accompanied by varying amounts of deposited extracellular matrix and differences in cell-to-cell junctions within each biofilm. Importantly, all Scl1-negative mutants examined showed significantly decreased ability to form biofilm in vitro. Furthermore, the Scl1 protein expressed on the surface of a heterologous host, Lactococcus lactis, was sufficient to induce biofilm formation by this organism.
conclusionsOverall, this work (i) identifies variations in biofilm formation capacity among pathogenically different GAS strains, (ii) identifies GAS surface properties that may aid in biofilm stability and, (iii) establishes that the Scl1 surface protein is an important determinant of GAS biofilm, which is sufficient to enable biofilm formation in the heterologous host Lactococcus. In summary, the GAS surface adhesin Scl1 may have an important role in biofilm-associated pathogenicity.
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Registered trials
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