ArticleBMC biotechnology2011
Targeted sequencing library preparation by genomic DNA circularization.
Article in BMC biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- High-resolution targeted bisulfite sequencing reveals blood cell type-specific DNA methylation patterns in IL13 and ORMDL3.Clinical epigenetics · 2021Observational
- BART-Seq: cost-effective massively parallelized targeted sequencing for genomics, transcriptomics, and single-cell analysis.Genome biology · 2019Article
- Efficient genome-wide genotyping strategies and data integration in crop plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Review
- Metabolic profiling and novel plasma biomarkers for predicting survival in epithelial ovarian cancer.Oncotarget · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
backgroundFor next generation DNA sequencing, we have developed a rapid and simple approach for preparing DNA libraries of targeted DNA content. Current protocols for preparing DNA for next-generation targeted sequencing are labor-intensive, require large amounts of starting material, and are prone to artifacts that result from necessary PCR amplification of sequencing libraries. Typically, sample preparation for targeted NGS is a two-step process where (1) the desired regions are selectively captured and (2) the ends of the DNA molecules are modified to render them compatible with any given NGS sequencing platform.
resultsIn this proof-of-concept study, we present an integrated approach that combines these two separate steps into one. Our method involves circularization of a specific genomic DNA molecule that directly incorporates the necessary components for conducting sequencing in a single assay and requires only one PCR amplification step. We also show that specific regions of the genome can be targeted and sequenced without any PCR amplification.
conclusionWe anticipate that these rapid targeted libraries will be useful for validation of variants and may have diagnostic application.
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Registered trials
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