ArticleProceedings of the National Academy of Sciences of the United States of America2011
Bleaching/blinking assisted localization microscopy for superresolution imaging using standard fluorescent molecules.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed, 207 citations in OpenAlex.
- Spontaneous and indefinite blinking in upconverting nanoparticles for ångström-precision multicolour super-resolution imaging.Nature nanotechnology · 2026Article
- Advancements in Single-Molecule Fluorescence Detection Techniques and Their Expansive Applications in Drug Discovery and Neuroscience.Biosensors · 2025Review
- Resolving viral structural complexity by super-resolution microscopy.Archives of virology · 2024Review
- Bright and stable monomeric green fluorescent protein derived from StayGold.Nature methods · 2024Article
- Photochemical Mechanisms of Fluorophores Employed in Single-Molecule Localization Microscopy.Angewandte Chemie (International ed. in English) · 2023Review
- Direct observation of heterogeneous formation of amyloid spherulites in real-time by super-resolution microscopy.Communications biology · 2022Article
- Centromere Tension Measurement in Budding Yeast Mitosis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Multimode Time-Resolved Superresolution Microscopy Revealing Chain Packing and Anisotropic Single Carrier Transport in Conjugated Polymer Nanowires.Nano letters · 2021Article
- Challenges facing quantitative large-scale optical super-resolution, and some simple solutions.iScience · 2021Review
- Time-correlated single molecule localization microscopy enhances resolution and fidelity.Scientific reports · 2020Article
- Dual-Mode Superresolution Imaging Using Charge Transfer Dynamics in Semiconducting Polymer Dots.Angewandte Chemie (International ed. in English) · 2020Article
- Modulation and study of photoblinking behavior in dye doped silver-silica core-shell nanoparticles for localization super-resolution microscopy.Nanotechnology · 2019Article
- Optimised insert design for improved single-molecule imaging and quantification through CRISPR-Cas9 mediated knock-in.Scientific reports · 2019Article
- Dynamic polyhedral actomyosin lattices remodel micron-scale curved membranes during exocytosis in live mice.Nature cell biology · 2019Article
- A Novel Microscopic Assay Reveals Heterogeneous Regulation of Local Endothelial Barrier Function.Biophysical journal · 2019Article
- Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification.Nature communications · 2019Article
- Photoregulated fluxional fluorophores for live-cell super-resolution microscopy with no apparent photobleaching.Nature communications · 2019Article
- Lightsheet localization microscopy enables fast, large-scale, and three-dimensional super-resolution imaging.Communications biology · 2019Article
- Switchable Fluorophores for Single-Molecule Localization Microscopy.Chemical reviews · 2018Review
- Artifact-free high-density localization microscopy analysis.Nature methods · 2018Article
17 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Superresolution imaging techniques based on the precise localization of single molecules, such as photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM), achieve high resolution by fitting images of single fluorescent molecules with a theoretical Gaussian to localize them with a precision on the order of tens of nanometers. PALM/STORM rely on photoactivated proteins or photoswitching dyes, respectively, which makes them technically challenging. We present a simple and practical way of producing point localization-based superresolution images that does not require photoactivatable or photoswitching probes. Called bleaching/blinking assisted localization microscopy (BaLM), the technique relies on the intrinsic bleaching and blinking behaviors characteristic of all commonly used fluorescent probes. To detect single fluorophores, we simply acquire a stream of fluorescence images. Fluorophore bleach or blink-off events are detected by subtracting from each image of the series the subsequent image. Similarly, blink-on events are detected by subtracting from each frame the previous one. After image subtractions, fluorescence emission signals from single fluorophores are identified and the localizations are determined by fitting the fluorescence intensity distribution with a theoretical Gaussian. We also show that BaLM works with a spectrum of fluorescent molecules in the same sample. Thus, BaLM extends single molecule-based superresolution localization to samples labeled with multiple conventional fluorescent probes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.