ArticleMethods in molecular biology (Clifton, N.J.)2012
Isotope labeling in insect cells.
Article in Methods in molecular biology (Clifton, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Human cells for human proteins: Isotope labeling in mammalian cells in suspension for functional NMR studies.Protein science : a publication of the Protein Society · 2026Article
- Cupriavidus necator, an alternative source for isotopic enrichment of proteins expressed in insect cells for NMR investigations.Journal of biomolecular NMR · 2026Article
- Automated Assignment ofJournal of the American Society for Mass Spectrometry · 2024Article
- Uniform [Journal of biomolecular NMR · 2024Article
- Glutamine-free mammalian expression of recombinant glycoproteins with uniform isotope labeling: an application for NMR analysis of pharmaceutically relevant Fc glycoforms of human immunoglobulin G1.Journal of biomolecular NMR · 2022Article
- Proton Detected Solid-State NMR of Membrane Proteins at 28 Tesla (1.2 GHz) and 100 kHz Magic-Angle Spinning.Biomolecules · 2021Article
- NMR Spectroscopic Characterization of the C-Mannose Conformation in a Thrombospondin Repeat Using a Selective Labeling Approach.Angewandte Chemie (International ed. in English) · 2020Article
- Silkworm Pupae Function as Efficient Producers of Recombinant Glycoproteins with Stable-Isotope Labeling.Biomolecules · 2020Article
- Production of isotope-labeled proteins in insect cells for NMR.Journal of biomolecular NMR · 2018Review
- Stable isotope labeling approaches for NMR characterization of glycoproteins using eukaryotic expression systems.Journal of biomolecular NMR · 2018Article
- Permeating disciplines: Overcoming barriers between molecular simulations and classical structure-function approaches in biological ion transport.Biochimica et biophysica acta. Biomembranes · 2018Review
- Solid state NMR of isotope labelled murine fur: a powerful tool to study atomic level keratin structure and treatment effects.Journal of biomolecular NMR · 2016Article
- Efficient production of (2)H, (13)C, (15)N-enriched industrial enzyme Rhizopus chinensis lipase with native disulfide bonds.Microbial cell factories · 2016Article
- Preparation of highly and generally enriched mammalian tissues for solid state NMR.Journal of biomolecular NMR · 2015Article
- Stable isotope labeling of glycoprotein expressed in silkworms using immunoglobulin G as a test molecule.Journal of biomolecular NMR · 2015Article
- Sparse labeling of proteins: structural characterization from long range constraints.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2014Review
- Structural biology of TRP channels.Handbook of experimental pharmacology · 2014Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Recent years have seen remarkable progress in applying nuclear magnetic resonance (NMR) spectroscopy to proteins that have traditionally been difficult to study due to issues with folding, posttranslational modification, and expression levels or combinations thereof. In particular, insect cells have proved useful in allowing large quantities of isotope-labeled, functional proteins to be obtained and purified to homogeneity, allowing study of their structures and dynamics by using NMR. Here, we provide protocols that have proven successful in such endeavors.
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Registered trials
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