ArticleJournal of biomolecular NMR2012
Backbone assignment of perdeuterated proteins using long-range H/C-dipolar transfers.
Article in Journal of biomolecular NMR, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
7 citing papers in PubMed, 20 citations in OpenAlex.
- Ultrafast Magic Angle Spinning Solid-State NMR Spectroscopy: Advances in Methodology and Applications.Chemical reviews · 2023Review
- Sequential backbone assignment based on dipolar amide-to-amide correlation experiments.Journal of biomolecular NMR · 2015Article
- MAS solid state NMR of proteins: simultaneous ¹⁵N- ¹³CA and ¹⁵N- ¹³CO dipolar recoupling via low-power symmetry-based RF pulse schemes.Journal of biomolecular NMR · 2015Article
- Towards automatic protein backbone assignment using proton-detected 4D solid-state NMR data.Journal of biomolecular NMR · 2014Article
- High-resolution proton CRAMPS NMR using narrowband analog filters and postponed data acquisition.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2013Article
- Solid state NMR of proteins at high MAS frequencies: symmetry-based mixing and simultaneous acquisition of chemical shift correlation spectra.Journal of biomolecular NMR · 2012Article
- Solid-state NMR analysis of membrane proteins and protein aggregates by proton detected spectroscopy.Journal of biomolecular NMR · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For micro-crystalline proteins, solid-state nuclear magnetic resonance spectroscopy of perdeuterated samples can provide spectra of unprecedented quality. Apart from allowing to detect sparsely introduced protons and thereby increasing the effective resolution for a series of sophisticated techniques, deuteration can provide extraordinary coherence lifetimes--obtainable for all involved nuclei virtually without decoupling and enabling the use of scalar magnetization transfers. Unfortunately, for fibrillar or membrane-embedded proteins, significantly shorter transverse relaxation times have been encountered as compared to micro-crystalline proteins despite an identical sample preparation, calling for alternative strategies for resonance assignment. In this work we propose an approach towards sequential assignment of perdeuterated proteins based on long-range (1)H/(13)C Cross Polarization transfers. This strategy gives rise to H/N-separated correlations involving C(α), C(β), and CO chemical shifts of both, intra- and interresidual contacts, and thus connecting adjacent residues independent of transverse relaxation times.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.