ArticlePlant molecular biology2012
Targeted transcriptional repression using a chimeric TALE-SRDX repressor protein.
Article in Plant molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed, 152 citations in OpenAlex.
- A dual-platform toolkit for transient gene expression and genome editing in rubber dandelion (aBIOTECH · 2026Article
- Synthetic transcriptional repression systems in plants.Plant cell reports · 2026Review
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- An AINTEGUMENTA phosphoswitch controls bilateral stem cell activity during secondary growth.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- CRISPRi-based circuits to control gene expression in plants.Nature biotechnology · 2025Article
- Recent advances in designing synthetic plant regulatory modules.Frontiers in plant science · 2025Review
- Systems for Targeted Silencing of Gene Expression and Their Application in Plants and Animals.International journal of molecular sciences · 2024Review
- Dissection of the IDA promoter identifies WRKY transcription factors as abscission regulators in Arabidopsis.Journal of experimental botany · 2024Article
- Advancements in genome editing tools for genetic studies and crop improvement.Frontiers in plant science · 2024Review
- Transcriptional and post-transcriptional controls for tuning gene expression in plants.Current opinion in plant biology · 2023Review
- Development of Designer Transcription Activator-Like Effector-Based Plant Growth Regulator for Higher Yield in Rice.Frontiers in plant science · 2022Article
- CRISPR/dCas9-Based Systems: Mechanisms and Applications in Plant Sciences.Plants (Basel, Switzerland) · 2021Review
- Deciphering Plant Chromatin Regulation via CRISPR/dCas9-Based Epigenome Engineering.Epigenomes · 2021Review
- Chromatin Manipulation and Editing: Challenges, New Technologies and Their Use in Plants.International journal of molecular sciences · 2021Review
- The genetic arms race between plant and Xanthomonas: lessons learned from TALE biology.Science China. Life sciences · 2021Review
- Genetic circuit characterization by inferring RNA polymerase movement and ribosome usage.Nature communications · 2020Article
- Review
- Biosystems Design to Accelerate CBiodesign research · 2020Review
- CRISPR/dCas9 platforms in plants: strategies and applications beyond genome editing.Plant biotechnology journal · 2020Review
- Changes to the core and flanking sequences of G-box elements lead to increases and decreases in gene expression in both native and synthetic soybean promoters.Plant biotechnology journal · 2019Article
11 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcriptional activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria when they infect plants. TALEs contain a modular DNA binding domain that can be easily engineered to bind any sequence of interest, and have been used to provide user-selected DNA-binding modules to generate chimeric nucleases and transcriptional activators in mammalian cells and plants. Here we report the use of TALEs to generate chimeric sequence-specific transcriptional repressors. The dHax3 TALE was used as a scaffold to provide a DNA-binding module fused to the EAR-repression domain (SRDX) to generate a chimeric repressor that targets the RD29A promoter. The dHax3.SRDX protein efficiently repressed the transcription of the RD29A::LUC transgene and endogenous RD29A gene in Arabidopsis. Genome wide expression profiling showed that the chimeric repressor also inhibited the expression of several other genes that contain the designer TALE-target sequence in their promoters. Our data suggest that TALEs can be used to generate chimeric repressors to specifically repress the transcription of genes of interest in plants. This sequence-specific transcriptional repression by direct on promoter effector technology is a powerful tool for functional genomics studies and biotechnological applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.