ArticleThe Journal of biological chemistry2012
REF4 and RFR1, subunits of the transcriptional coregulatory complex mediator, are required for phenylpropanoid homeostasis in Arabidopsis.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
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- Insights into the early transcriptomic response against watermelon mosaic virus in melon.BMC plant biology · 2024Article
- Uncovering tomato candidate genes associated with drought tolerance using Solanum pennellii introgression lines.PloS one · 2023Article
- DNA methylation in clonal duckweed (Lemna minor L.) lineages reflects current and historical environmental exposures.Molecular ecology · 2023Article
- Transcript and metabolite network perturbations in lignin biosynthetic mutants of Arabidopsis.Plant physiology · 2022Article
- H-lignin can be deposited independently of CINNAMYL ALCOHOL DEHYDROGENASE C and D in Arabidopsis.Plant physiology · 2022Article
- Manipulation of Lignin Monomer Composition Combined with the Introduction of Monolignol Conjugate Biosynthesis Leads to Synergistic Changes in Lignin Structure.Plant & cell physiology · 2022Article
- The Mediator Complex: A Central Coordinator of Plant Adaptive Responses to Environmental Stresses.International journal of molecular sciences · 2022Review
- Transcriptome and metabolome analyses revealed that narrowband 280 and 310 nm UV-B induce distinctive responses in Arabidopsis.Scientific reports · 2022Article
- GIpred: a computational tool for prediction of GIGANTEA proteins using machine learning algorithm.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Multi-omic characterization of bifunctional peroxidase 4-coumarate 3-hydroxylase knockdown inFrontiers in plant science · 2022Article
- Transcriptional Coactivators: Driving Force of Plant Immunity.Frontiers in plant science · 2022Review
- Towards a Cardoon (International journal of molecular sciences · 2021Article
- A genome-scale TF-DNA interaction network of transcriptional regulation of Arabidopsis primary and specialized metabolism.Molecular systems biology · 2021Article
- Tailoring renewable materials via plant biotechnology.Biotechnology for biofuels · 2021Review
- Metabolic source isotopic pair labeling and genome-wide association are complementary tools for the identification of metabolite-gene associations in plants.The Plant cell · 2021Article
- Arabidopsis MED18 Interaction With RNA Pol IV and V Subunit NRPD2a in Transcriptional Regulation of Plant Immune Responses.Frontiers in plant science · 2021Article
- Syringic Acid Alleviates Cesium-Induced Growth Defect inInternational journal of molecular sciences · 2020Article
- Mapping the Gene Expression Spectrum of Mediator Subunits in Response to Viroid Infection in Plants.International journal of molecular sciences · 2020Article
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9 authors.
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Abstract
The plant phenylpropanoid pathway produces an array of metabolites that impact human health and the utility of feed and fiber crops. We previously characterized several Arabidopsis thaliana mutants with dominant mutations in REDUCED EPIDERMAL FLUORESCENCE 4 (REF4) that cause dwarfing and decreased accumulation of phenylpropanoids. In contrast, ref4 null plants are of normal stature and have no apparent defect in phenylpropanoid biosynthesis. Here we show that disruption of both REF4 and its paralog, REF4-RELATED 1 (RFR1), results in enhanced expression of multiple phenylpropanoid biosynthetic genes, as well as increased accumulation of numerous downstream products. We also show that the dominant ref4-3 mutant protein interferes with the ability of the PAP1/MYB75 transcription factor to induce the expression of PAL1 and drive anthocyanin accumulation. Consistent with our experimental results, both REF4 and RFR1 have been shown to physically associate with the conserved transcriptional coregulatory complex, Mediator, which transduces information from cis-acting DNA elements to RNA polymerase II at the core promoter. Taken together, our data provide critical genetic support for a functional role of REF4 and RFR1 in the Mediator complex, and for Mediator in the maintenance of phenylpropanoid homeostasis. Finally, we show that wild-type RFR1 substantially mitigates the phenotype of the dominant ref4-3 mutant, suggesting that REF4 and RFR1 may compete with one another for common binding partners or for occupancy in Mediator. Determining the functions of diverse Mediator subunits is essential to understand eukaryotic gene regulation, and to facilitate rational manipulation of plant metabolic pathways to better suit human needs.
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