ArticleThe Journal of biological chemistry2012
Activity and regulation of archaeal DNA alkyltransferase: conserved protein involved in repair of DNA alkylation damage.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 31 citations in OpenAlex.
- Click biology highlights the opportunities from reliable biological reactions.Nature chemical biology · 2025Review
- Alkylated DNA repair by a novel HhH-GPD family protein from Crenarchaea.Nucleic acids research · 2025Article
- The DNA Alkyltransferase Family of DNA Repair Proteins: Common Mechanisms, Diverse Functions.International journal of molecular sciences · 2023Review
- Archaeal DNA alkylation repair conducted by DNA glycosylase and methyltransferase.Applied microbiology and biotechnology · 2023Review
- First thermostable CLIP-Computational and structural biotechnology journal · 2022Article
- The SNAP-Journal of enzyme inhibition and medicinal chemistry · 2021Article
- Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag.Biochemistry · 2021Article
- Folding-upon-Repair DNA Nanoswitches for Monitoring the Activity of DNA Repair Enzymes.Angewandte Chemie (International ed. in English) · 2021Article
- Review
- A journey down to hell: new thermostable protein-tags for biotechnology at high temperatures.Extremophiles : life under extreme conditions · 2020Article
- An AGT-based protein-tag system for the labelling and surface immobilization of enzymes on E. coli outer membrane.Journal of enzyme inhibition and medicinal chemistry · 2019Article
- The DNA Alkylguanine DNA Alkyltransferase-2 (AGT-2) Of Caenorhabditis Elegans Is Involved In Meiosis And Early Development Under Physiological Conditions.Scientific reports · 2019Article
- Optimization of an In Vitro Transcription/Translation System Based onArchaea (Vancouver, B.C.) · 2019Article
- Every OGT Is Illuminated … by Fluorescent and Synchrotron Lights.International journal of molecular sciences · 2017Review
- In vivo and in vitro protein imaging in thermophilic archaea by exploiting a novel protein tag.PloS one · 2017Article
- Identification of a mismatch-specific endonuclease in hyperthermophilic Archaea.Nucleic acids research · 2016Article
- A novel thermostable protein-tag: optimization of the Sulfolobus solfataricus DNA- alkyl-transferase by protein engineering.Extremophiles : life under extreme conditions · 2016Article
- Structure-function relationships governing activity and stability of a DNA alkylation damage repair thermostable protein.Nucleic acids research · 2015Article
- Insight into the cellular involvement of the two reverse gyrases from the hyperthermophilic archaeon Sulfolobus solfataricus.BMC molecular biology · 2014Article
- Genome stability: recent insights in the topoisomerase reverse gyrase and thermophilic DNA alkyltransferase.Extremophiles : life under extreme conditions · 2014Review
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agents that form methylation adducts in DNA are highly mutagenic and carcinogenic, and organisms have evolved specialized cellular pathways devoted to their repair, including DNA alkyltransferases. These are proteins conserved in eucarya, bacteria and archaea, acting by a unique reaction mechanism, which leads to direct repair of DNA alkylation damage and irreversible protein alkylation. The alkylated form of DNA alkyltransferases is inactive, and in eukaryotes, it is rapidly directed to degradation. We report here in vitro and in vivo studies on the DNA alkyltransferase from the thermophilic archaeon Sulfolobus solfataricus (SsOGT). The development of a novel, simple, and sensitive fluorescence-based assay allowed a careful characterization of the SsOGT biochemical and DNA binding activities. In addition, transcriptional and post-translational regulation of SsOGT by DNA damage was studied. We show that although the gene transcription is induced by alkylating agent treatment, the protein is degraded in vivo by an alkylation-dependent mechanism. These experiments suggest a striking conservation, from archaea to humans, of this important pathway safeguarding genome stability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.