ArticleTissue engineering. Part C, Methods2012
Evaluation of the cell viability of human Wharton's jelly stem cells for use in cell therapy.
Article in Tissue engineering. Part C, Methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 50 citations in OpenAlex.
- Dentinogenic Effect of BMP-7 on Wharton's Jelly Mesenchymal Stem Cells Cultured in Decellularized Dental Pulp.International journal of molecular sciences · 2025Article
- Expansion of human umbilical cord derived mesenchymal stem cells in regenerative medicine.World journal of stem cells · 2024Article
- Combined Placental Mesenchymal Stem Cells with Guided Nanoparticles Effective Against Diabetic Nephropathy in Mouse Model.International journal of nanomedicine · 2024Article
- Review
- A novel 3D biofabrication strategy to improve cell proliferation and differentiation of human Wharton's jelly mesenchymal stromal cells for cell therapy and tissue engineering.Frontiers in bioengineering and biotechnology · 2023Article
- Generation of a novel model of bioengineered human oral mucosa with increased vascularization potential.Journal of periodontal research · 2021Article
- Ex Vivo Generation and Characterization of Human Hyaline and Elastic Cartilaginous Microtissues for Tissue Engineering Applications.Biomedicines · 2021Article
- Successful development and clinical translation of a novel anterior lamellar artificial cornea.Journal of tissue engineering and regenerative medicine · 2019Article
- Wharton's jelly-derived mesenchymal cells as a new source for the generation of microtissues for tissue engineering applications.Histochemistry and cell biology · 2018Article
- Impedance-based cellular assays for regenerative medicine.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- Tumor Necrosis Factor-α Induced Protein 8: Pathophysiology, Clinical Significance, and Regulatory Mechanism.International journal of biological sciences · 2018Review
- High Sensitivity of Human Adipose Stem Cells to Differentiate into Myofibroblasts in the Presence ofStem cells international · 2017Article
- Encapsulation of human elastic cartilage-derived chondrocytes in nanostructured fibrin-agarose hydrogels.Histochemistry and cell biology · 2017Article
- Stem Cells Applications in Regenerative Medicine and Disease Therapeutics.International journal of cell biology · 2016Review
- Different isolation methods alter the gene expression profiling of adipose derived stem cells.International journal of medical sciences · 2014Article
- Wharton's jelly stem cells: a novel cell source for oral mucosa and skin epithelia regeneration.Stem cells translational medicine · 2013Article
- The therapeutic potential, challenges and future clinical directions of stem cells from the Wharton's jelly of the human umbilical cord.Stem cell reviews and reports · 2013Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human umbilical cord Wharton's jelly stem cells (HWJSCs) are gaining attention as a possible clinical source of mesenchymal stem cells for cell therapy and tissue engineering due to their high accessibility, expansion potential, and plasticity. We employed a combination of highly sensitive techniques to determine the average cell viability levels and proliferation capabilities of 10 consecutive cell passages of cultured HWJSCs and then used RNA microarrays to identify genes associated with changes in cell viability levels. We found an initial decrease in cell viability from the first to the third cell passage followed by an increase until the sixth passage and a final decrease from the sixth to tenth cell passages. The highest cell viability levels corresponded to the fifth and sixth passages. The intracellular ionic contents of potassium, sodium, and chlorine suggest that the lower cell viability levels at passages 2, 3, and 8-10 may be associated with apoptotic cell death. In fact, gene expression analysis revealed that the average cell viability was significantly associated with genes with a function in apoptotic cell death, especially pro-apoptotic FASTKD2, BNIP3L genes and anti-apoptotic TNFAIP8 and BCL2L2 genes. This correlation with both pro-apoptotic and anti-apoptotic genes suggests that there may be a complex live-death equilibrium in cultured HWJSCs kept in culture for multiple cell passages. In this study, the highest cell viability levels corresponded to the fifth and sixth HWJSC passages, suggesting that these passages should be preferentially employed in cell therapy or tissue engineering protocols using this cell type.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.