ArticleTissue engineering. Part C, Methods2012
Comparative assessment of detergent-based protocols for mouse lung de-cellularization and re-cellularization.
Article in Tissue engineering. Part C, Methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers.
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Who cites it
94 citing papers in PubMed, 185 citations in OpenAlex.
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- Towards Safety and Regulation Criteria for Clinical Applications of Decellularized Organ-Derived Matrices.Bioengineering (Basel, Switzerland) · 2025Review
- Decellularisation and Characterisation of Porcine Pleura as Bioscaffolds in Tissue Engineering.Journal of tissue engineering and regenerative medicine · 2024Article
- Detergent-Based Decellularization for Anisotropic Cardiac-Specific Extracellular Matrix Scaffold Generation.Biomimetics (Basel, Switzerland) · 2023Article
- Regional and disease-specific glycosaminoglycan composition and function in decellularized human lung extracellular matrix.Acta biomaterialia · 2023Article
- Bioengineering and Clinical Translation of Human Lung and its Components.Advanced biology · 2023Review
- What is the need and why is it time for innovative models for understanding lung repair and regeneration?Frontiers in pharmacology · 2023Review
- Analysis of decellularized mouse liver fragment and its recellularization with human endometrial mesenchymal cells as a candidate for clinical usage.Progress in biomaterials · 2022Article
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- Perfusion decellularization for vascularized composite allotransplantation.SAGE open medicine · 2022Review
- Bioengineering lungs: An overview of current methods, requirements, and challenges for constructing scaffolds.Frontiers in bioengineering and biotechnology · 2022Review
- Cell Inertia: Predicting Cell Distributions in Lung Vasculature to Optimize Re-endothelialization.Frontiers in bioengineering and biotechnology · 2022Article
- Recellularization of Native Tissue Derived Acellular Scaffolds with Mesenchymal Stem Cells.Cells · 2021Review
- Building a stem cell-based primate uterus.Communications biology · 2021Review
34 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Several different detergent-based methods are currently being explored for de-cellularizing whole lungs for subsequent use as three-dimensional scaffolds for ex vivo lung tissue generation. However, it is not yet clear which of these methods may provide a scaffold that best supports re-cellularization and generation of functional lung tissue. Notably, the detergents used for de-cellularization activate matrix metalloproteinases that can potentially degrade extracellular matrix (ECM) proteins important for subsequent binding and growth of cells inoculated into the de-cellularized scaffolds. We assessed gelatinase activation and the histologic appearance, protein composition, and lung mechanics of the end product scaffolds produced with three different detergent-based de-cellularization methods utilizing either Triton-X 100/sodium deoxycholate (Triton/SDC), sodium dodecyl sulfate (SDS), or 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS). There were significant differences both in gelatinase activation and in the retention of ECM and other intracellular proteins, assessed by immunohistochemistry, mass spectrometry, and western blotting as well as in airways resistance and elastance of lungs de-cellularized with the different methods. However, despite these differences, binding and initial growth following intratracheal inoculation with either bone marrow-derived mesenchymal stromal cells or with C10 mouse lung epithelial cells was similar between lungs de-cellularized with each method. Therefore despite differences in the structural composition of the de-cellularized lungs, initial re-cellularization does not appear significantly different between the three de-cellularization approaches studied.
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