ArticleBacteriophage2011
Genomic and proteomic characterization of the large Myoviridae bacteriophage ϕTMA of the extreme thermophile Thermus thermophilus.
Article in Bacteriophage, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Residue-level determinants of the thermal stability of the extremophilic Ts2631 endolysin.Scientific reports · 2026Article
- Multiomics Approaches for Bacteriophage-Based Biocontrol Applications: A Review of Metabolomics, Transcriptomics, and Proteomics.BioMed research international · 2026Review
- Review
- Rapid water flow triggers long-distance positive rheotaxis for thermophilic bacteria.The ISME journal · 2025Article
- Precise and Accurate DNA-3'/5-Ends Polishing withInternational journal of molecular sciences · 2024Article
- Metagenomics unravel distinct taxonomic and functional diversities between terrestrial and aquatic biomes.iScience · 2024Article
- The Diversity of Bacteriophages in Hot Springs.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Functional biology and biotechnology of thermophilic viruses.Essays in biochemistry · 2023Review
- Potential Applications of Thermophilic Bacteriophages in One Health.International journal of molecular sciences · 2023Review
- Molecular Characterization of a DNA Polymerase fromInternational journal of molecular sciences · 2022Article
- Crystal structure and initial characterization of a novel archaeal-like Holliday junction-resolving enzyme from Thermus thermophilus phage Tth15-6.Acta crystallographica. Section D, Structural biology · 2022Article
- A novel thermophilic Aeribacillus bacteriophage AP45 isolated from the Valley of Geysers, Kamchatka: genome analysis suggests the existence of a new genus within the Siphoviridae family.Extremophiles : life under extreme conditions · 2019Article
- Natural diversity of CRISPR spacers of Thermus: evidence of local spacer acquisition and global spacer exchange.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Biogeography and taxonomic overview of terrestrial hot spring thermophilic phages.Extremophiles : life under extreme conditions · 2018Review
- Foreword to 'Multiscale structural biology: biophysical principles and mechanisms underlying the action of bio-nanomachines', a special issue in Honour of Fumio Arisaka's 70th birthday.Biophysical reviews · 2018Article
- Intriguing Interaction of Bacteriophage-Host Association: An Understanding in the Era of Omics.Frontiers in microbiology · 2017Review
- Complete Genome Analysis ofFrontiers in microbiology · 2017Article
- Physiological Properties and Genome Structure of the Hyperthermophilic Filamentous Phage φOH3 Which Infects Thermus thermophilus HB8.Frontiers in microbiology · 2016Article
- Complete Genome Sequence of Thermus aquaticus Y51MC23.PloS one · 2015Article
Corrections and comments
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Authors and funding
15 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A lytic phage, designated as ϕTMA, was isolated from a Japanese hot spring using Thermus thermophilus HB27 as an indicator strain. Electron microscopic examination showed that ϕTMA had an icosahedral head and a contractile tail. The circular double-stranded DNA sequence of ϕTMA was 151,483 bp in length, and its organization was essentially same as that of ϕYS40 except that the ϕTMA genome contained genes for a pair of transposase and resolvase, and a gene for a serine to asparagine substituted ortholog of the protein involved in the initiation of the ϕYS40 genomic DNA synthesis. The different host specificities of ϕTMA and ϕYS40 could be explained by the sequence differences in the C-terminal regions of their distal tail fiber proteins. The ΔpilA knockout strains of T. thermophilus showed simultaneous loss of sensitivity to their cognate phages, pilus structure, twitching motility and competence for natural transformation, thus suggesting that the phage infection required the intact host pili. Pulsed-field gel electrophoresis analysis of the ϕTMA and ϕYS40 genomes revealed that the length of their DNA exceeded 200 kb, indicating that the terminal redundancy is more than 30% of the closed circular form. Proteomic analysis of the ϕTMA virion using a combination of N-terminal sequencing and mass spectrometric analysis of peptide fragments suggested that the maturation of several proteins involved in the phage assembly process was mediated by a trypsin-like protease. The gene order of the phage structural proteins was also discussed.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.