ArticlePloS one2011
Establishment of normal gut microbiota is compromised under excessive hygiene conditions.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers, 1 of them a synthesis that pooled it.
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Who cites it
70 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Plasticity of intestinal gene expression profile signatures reflected by nutritional interventions in piglets.BMC genomics · 2019Pooled it
- The role of human-pig interactions in modulating gut microbiota, stress, and performance.Porcine health management · 2025Article
- Effect of Maternal Probiotic and Piglet Dietary Tryptophan Level on Performance and Piglet Intestinal Health Parameters Pre-Weaning.Microorganisms · 2025Article
- New perspectives on microbiome-dependent gut-brain pathways for the treatment of depression with gastrointestinal symptoms: from bench to bedside.Journal of Zhejiang University. Science. B · 2025Review
- Do high-protein diets have the potential to reduce gut barrier function in a sex-dependent manner?European journal of nutrition · 2024Article
- Maternal versus environmental contributions to the piglet pioneer microbiome.Reproduction & fertility · 2024Article
- A comparative study of the bacterial diversity and composition of nursery piglets' oral fluid, feces, and housing environment.Scientific reports · 2024Article
- The Effect of Maternal Probiotic or Synbiotic Supplementation on Sow and Offspring Gastrointestinal Microbiota, Health, and Performance.Animals : an open access journal from MDPI · 2023Review
- A comparison of the infant gut microbiome before versus after the start of the covid-19 pandemic.Scientific reports · 2023Article
- Association between Residential Greenness and Human Microbiota: Evidence from Multiple Countries.Environmental health perspectives · 2023Article
- The Potential Role of Microorganisms on Enteric Nervous System Development and Disease.Biomolecules · 2023Review
- Post-weaning diarrhea in pigs from a single Danish production herd was not associated with the pre-weaning fecal microbiota composition and diversity.Frontiers in microbiology · 2023Article
- An insight into the commercial piglet's microbial gut colonization: from birth towards weaning.Animal microbiome · 2022Article
- Interactions between gut microbiota and berberine, a necessary procedure to understand the mechanisms of berberine.Journal of pharmaceutical analysis · 2022Review
- Influence of Mycoplasma hyopneumoniae natural infection on the respiratory microbiome diversity of finishing pigs.Veterinary research · 2022Article
- Effects of iron deficiency and iron supplementation at the host-microbiota interface: Could a piglet model unravel complexities of the underlying mechanisms?Frontiers in nutrition · 2022Review
- Porcine Models of the Intestinal Microbiota: The Translational Key to Understanding How Gut Commensals Contribute to Gastrointestinal Disease.Frontiers in veterinary science · 2022Review
- Disinfection of Maternal Environments Is Associated with Piglet Microbiome Composition from Birth to Weaning.mSphere · 2021Article
- The gut microbiota and microbial metabolites are associated with tail biting in pigs.Scientific reports · 2021Article
- Supplementation of Lactobacillus early in life alters attention bias to threat in piglets.Scientific reports · 2021Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEarly gut colonization events are purported to have a major impact on the incidence of infectious, inflammatory and autoimmune diseases in later life. Hence, factors which influence this process may have important implications for both human and animal health. Previously, we demonstrated strong influences of early-life environment on gut microbiota composition in adult pigs. Here, we sought to further investigate the impact of limiting microbial exposure during early life on the development of the pig gut microbiota. METHODOLOGY/PRINCIPAL
findingsOutdoor- and indoor-reared animals, exposed to the microbiota in their natural rearing environment for the first two days of life, were transferred to an isolator facility and adult gut microbial diversity was analyzed by 16S rRNA gene sequencing. From a total of 2,196 high-quality 16S rRNA gene sequences, 440 phylotypes were identified in the outdoor group and 431 phylotypes in the indoor group. The majority of clones were assigned to the four phyla Firmicutes (67.5% of all sequences), Proteobacteria (17.7%), Bacteroidetes (13.5%) and to a lesser extent, Actinobacteria (0.1%). Although the initial maternal and environmental microbial inoculum of isolator-reared animals was identical to that of their naturally-reared littermates, the microbial succession and stabilization events reported previously in naturally-reared outdoor animals did not occur. In contrast, the gut microbiota of isolator-reared animals remained highly diverse containing a large number of distinct phylotypes. CONCLUSIONS/SIGNIFICANCE: The results documented here indicate that establishment and development of the normal gut microbiota requires continuous microbial exposure during the early stages of life and this process is compromised under conditions of excessive hygiene.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.