ArticlePloS one2011
Engineering genetically encoded nanosensors for real-time in vivo measurements of citrate concentrations.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 71 citations in OpenAlex.
- Engineering theACS synthetic biology · 2026Article
- A Novel Biosensor for Ferrous Iron Developed via CoBiSe: A Computational Method for Rapid Biosensor Design.ACS sensors · 2026Article
- Activity-dependent citrate dynamics in neurons.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Unravelling molecular dynamics in living cells: Fluorescent protein biosensors for cell biology.Journal of microscopy · 2025Review
- A genetically encoded fluorescent sensor enables sensitive and specific detection of IDH mutant associated oncometabolite D-2-hydroxyglutarate.BMC cancer · 2025Article
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Engineered Bacteria as Living Biosensors in Dermal Tattoos.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Metabolic FRET sensors in intact organs: Applying spectral unmixing to acquire reliable signals.Biology open · 2023Article
- Recent progress in developing fluorescent probes for imaging cell metabolites.Biomedical materials (Bristol, England) · 2021Review
- Imaging Approaches for the Study of Metabolism in Real Time Using Genetically Encoded Reporters.Frontiers in cell and developmental biology · 2021Review
- Bacterial Vivisection: How Fluorescence-Based Imaging Techniques Shed a Light on the Inner Workings of Bacteria.Microbiology and molecular biology reviews : MMBR · 2020Review
- High-Performance Intensiometric Direct- and Inverse-Response Genetically Encoded Biosensors for Citrate.ACS central science · 2020Article
- Fluorescent Biosensors for Neuronal Metabolism and the Challenges of Quantitation.Current opinion in neurobiology · 2020Review
- Circularly Permuted Fluorescent Protein-Based Indicators: History, Principles, and Classification.International journal of molecular sciences · 2019Review
- Sex Differences in Intestinal Carbohydrate Metabolism Promote Food Intake and Sperm Maturation.Cell · 2019Article
- Engineering genetically encoded FRET-based nanosensors for real time display of arsenic (AsScientific reports · 2019Article
- Obesity-dependent CDK1 signaling stimulates mitochondrial respiration at complex I in pancreatic β-cells.The Journal of biological chemistry · 2019Article
- Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.Chemical reviews · 2018Review
- Dynamic single-cell NAD(P)H measurement reveals oscillatory metabolism throughout the E. coli cell division cycle.Scientific reports · 2018Article
- Imaging and spectroscopic approaches to probe brain energy metabolism dysregulation in neurodegenerative diseases.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2017Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Citrate is an intermediate in catabolic as well as biosynthetic pathways and is an important regulatory molecule in the control of glycolysis and lipid metabolism. Mass spectrometric and NMR based metabolomics allow measuring citrate concentrations, but only with limited spatial and temporal resolution. Methods are so far lacking to monitor citrate levels in real-time in-vivo. Here, we present a series of genetically encoded citrate sensors based on Förster resonance energy transfer (FRET). We screened databases for citrate-binding proteins and tested three candidates in vitro. The citrate binding domain of the Klebsiella pneumoniae histidine sensor kinase CitA, inserted between the FRET pair Venus/CFP, yielded a sensor highly specific for citrate. We optimized the peptide linkers to achieve maximal FRET change upon citrate binding. By modifying residues in the citrate binding pocket, we were able to construct seven sensors with different affinities spanning a concentration range of three orders of magnitude without losing specificity. In a first in vivo application we show that E. coli maintains the capacity to take up glucose or acetate within seconds even after long-term starvation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.