ArticleSensors (Basel, Switzerland)2011
Label-free electrochemical detection of the specific oligonucleotide sequence of dengue virus type 1 on pencil graphite electrodes.
Article in Sensors (Basel, Switzerland), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 50 citations in OpenAlex.
- Field-Based cDNA-Biosensor for Accurate Detection of Canine Distemper Virus in Tissue Samples.Applied biochemistry and biotechnology · 2025Article
- Biosensors for the detection of flaviviruses: A review.Synthetic and systems biotechnology · 2025Review
- Integration of nanobiosensors into organ-on-chip systems for monitoring viral infections.Nano convergence · 2024Review
- A Review of Detection Methods for Vancomycin-ResistantBiosensors · 2023Review
- Low-Cost High-Resolution Potentiostat for Electrochemical Detection of Nucleic Acids and Biomolecular Interactions.Micromachines · 2022Article
- Portable Sensing Devices for Detection of COVID-19: A Review.IEEE sensors journal · 2021Article
- Review
- A Sensitive and Selective Label-Free Electrochemical DNA Biosensor for the Detection of Specific Dengue Virus Serotype 3 Sequences.Sensors (Basel, Switzerland) · 2015Article
- Diagnostic tests for hepatitis C: recent trends in electrochemical immunosensor and genosensor analysis.World journal of gastroenterology · 2014Review
- Optimization of dengue immunoassay by label-free interferometric optical detection method.Sensors (Basel, Switzerland) · 2014Article
- Impedimetric DNA biosensor based on a nanoporous alumina membrane for the detection of the specific oligonucleotide sequence of dengue virus.Sensors (Basel, Switzerland) · 2013Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A biosensor that relies on the adsorption immobilization of the 18-mer single-stranded nucleic acid related to dengue virus gene 1 on activated pencil graphite was developed. Hybridization between the probe and its complementary oligonucleotides (the target) was investigated by monitoring guanine oxidation by differential pulse voltammetry (DPV). The pencil graphite electrode was made of ordinary pencil lead (type 4B). The polished surface of the working electrode was activated by applying a potential of 1.8 V for 5 min. Afterward, the dengue oligonucleotides probe was immobilized on the activated electrode by applying 0.5 V to the electrode in 0.5 M acetate buffer (pH 5.0) for 5 min. The hybridization process was carried out by incubating at the annealing temperature of the oligonucleotides. A time of five minutes and concentration of 1 μM were found to be the optimal conditions for probe immobilization. The electrochemical detection of annealing between the DNA probe (TS-1P) immobilized on the modified electrode, and the target (TS-1T) was achieved. The target could be quantified in a range from 1 to 40 nM with good linearity and a detection limit of 0.92 nM. The specificity of the electrochemical biosensor was tested using non-complementary sequences of dengue virus 2 and 3.
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