ArticleSensors (Basel, Switzerland)2010
Use of a multiplexed CMOS microarray to optimize and compare oligonucleotide binding to DNA probes synthesized or immobilized on individual electrodes.
Article in Sensors (Basel, Switzerland), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 28 citations in OpenAlex.
- Automated high-throughput DNA synthesis and assembly.Heliyon · 2024Review
- From Molecules to Molecular Surfaces. Exploiting the Interplay Between Organic Synthesis and Electrochemistry.Accounts of chemical research · 2020Review
- Electroorganic Synthesis and the Construction of Addressable Molecular Surfaces.ChemElectroChem · 2019Article
- Microelectrode Arrays and the Use of PEG-Functionalized Diblock Copolymer Coatings.Biosensors · 2014Article
- Site-selective chemistry and the attachment of peptides to the surface of a microelectrode array.Journal of the American Chemical Society · 2012Article
- Microelectrode array biosensor for studying carbohydrate-mediated interactions.Biosensors & bioelectronics · 2012Article
- Site-selective, cleavable linkers: quality control and the characterization of small molecules on microelectrode arrays.The Journal of organic chemistry · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The CombiMatrix microarray with 12,544 electrodes supports in situ electrochemical synthesis of user-defined DNA probes. As an alternative, we immobilized commercially synthesized DNA probes on individual electrodes coated with electropolymerized polypyrrole (Ppy). Hybridization was measured using a biotinylated target oligonucleotide and either Cy5-streptavidin and fluorescence detection or horseradish peroxidase-streptavidin and enzyme-enhanced electrochemical detection. Detection efficiencies were optimized by varying the deposition of the Ppy, the terminal groups on the DNA probes, and other factors that impacted fluorescence quenching and electrical conductivity. Optimized results were compared against those obtained using a microarray with the same DNA sequences synthesized in situ. Immobilized probes produced higher fluorescence signals, possibly by providing a greater stand off between the Cy5 on the target oligonucleotide and the quenching effects of the Ppy and the platinum electrode.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.