ArticleSensors (Basel, Switzerland)2010
Metaphase FISH on a chip: miniaturized microfluidic device for fluorescence in situ hybridization.
Article in Sensors (Basel, Switzerland), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 30 citations in OpenAlex.
- FISH and chips: a review of microfluidic platforms for FISH analysis.Medical microbiology and immunology · 2020Review
- Microfluidic Device for On-Chip Immunophenotyping and Cytogenetic Analysis of Rare Biological Cells.Cells · 2020Article
- Review
- MicroRNA amplification and detection technologies: opportunities and challenges for point of care diagnostics.Laboratory investigation; a journal of technical methods and pathology · 2019Review
- Microfluidics-assisted fluorescence in situ hybridization for advantageous human epidermal growth factor receptor 2 assessment in breast cancer.Laboratory investigation; a journal of technical methods and pathology · 2017Article
- Micro fluorescence in situ hybridization (μFISH) for spatially multiplexed analysis of a cell monolayer.Biomedical microdevices · 2016Article
- Fluorescence In situ Hybridization: Cell-Based Genetic Diagnostic and Research Applications.Frontiers in cell and developmental biology · 2016Review
- Next generation platforms for high-throughput biodosimetry.Radiation protection dosimetry · 2014Article
- A three dimensional thermoplastic microfluidic chip for robust cell capture and high resolution imaging.Biomicrofluidics · 2014Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence in situ Hybridization (FISH) is a major cytogenetic technique for clinical genetic diagnosis of both inherited and acquired chromosomal abnormalities. Although FISH techniques have evolved and are often used together with other cytogenetic methods like CGH, PRINS and PNA-FISH, the process continues to be a manual, labour intensive, expensive and time consuming technique, often taking over 3 5 days, even in dedicated labs. We have developed a novel microFISH device to perform metaphase FISH on a chip which overcomes many shortcomings of the current laboratory protocols. This work also introduces a novel splashing device for preparing metaphase spreads on a microscope glass slide, followed by a rapid adhesive tape-based bonding protocol leading to rapid fabrication of the microFISH device. The microFISH device allows for an optimized metaphase FISH protocol on a chip with over a 20-fold reduction in the reagent volume. This is the first demonstration of metaphase FISH on a microfluidic device and offers a possibility of automation and significant cost reduction of many routine diagnostic tests of genetic anomalies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.