Trial reportPloS one2011
Genomic DNA methylation changes in response to folic acid supplementation in a population-based intervention study among women of reproductive age.
Trial report in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 54 citations in OpenAlex.
- Feasibility Outcomes of a Pilot Randomized Clinical Trial to Increase Cruciferous and Green Leafy Vegetable Intake in Posttreatment Head and Neck Cancer Survivors.Journal of the Academy of Nutrition and Dietetics · 2019Trial
- Sex-Specific Associations between One-Carbon Metabolism Indices and Posttranslational Histone Modifications in Arsenic-Exposed Bangladeshi Adults.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2017Trial
- Consumption of methyl donor nutrients and incidence of obesity: is the association influenced by parent's obesity? Results of 4 years of follow-up of the CUME study.International journal of obesity (2005) · 2025Article
- Examining nutrition strategies to influence DNA methylation and epigenetic clocks: a systematic review of clinical trials.Frontiers in aging · 2024Review
- A Paternal Methylation Error in the Congenital Hydrocephalic Texas (H-Tx) Rat Is Partially Rescued with Natural Folate Supplements.International journal of molecular sciences · 2023Article
- Susceptibility to hypertension based onFrontiers in cardiovascular medicine · 2023Article
- Folate Carrier Deficiency Drives Differential Methylation and Enhanced Cellular Potency in the Neural Plate Border.Frontiers in cell and developmental biology · 2022Article
- Prenatal Multivitamin Use andInternational journal of environmental research and public health · 2020Article
- Role of Key Micronutrients from Nutrigenetic and Nutrigenomic Perspectives in Cancer Prevention.Medicina (Kaunas, Lithuania) · 2019Review
- Folate deficiency inhibits the PCP pathway and alters genomic methylation levels during embryonic development.Journal of cellular physiology · 2018Article
- Role of genetic and environmental factors in DNA methylation of lipid metabolism.Genes & diseases · 2018Review
- Blood triglyceride levels are associated with DNA methylation at the serine metabolism gene PHGDH.Scientific reports · 2017Article
- High-dose maternal folic acid supplementation before conception impairs reversal learning in offspring mice.Scientific reports · 2017Article
- Impact of a folic acid-enriched diet on urinary tract function in mice treated with testosterone and estradiol.American journal of physiology. Renal physiology · 2015Article
- Nutrition During Pregnancy Impacts Offspring's Epigenetic Status-Evidence from Human and Animal Studies.Nutrition and metabolic insights · 2015Review
- Article
- Hypomethylation of serum blood clot DNA, but not plasma EDTA-blood cell pellet DNA, from vitamin B12-deficient subjects.PloS one · 2013Article
- Epigenetic profiles in children with a neural tube defect; a case-control study in two populations.PloS one · 2013Article
- Article
- Micronutrient status and global DNA methylation in school-age children.Epigenetics · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Folate is a source of one-carbons necessary for DNA methylation, a critical epigenetic modification necessary for genomic structure and function. The use of supplemental folic acid is widespread however; the potential influence on DNA methylation is unclear. We measured global DNA methylation using DNA extracted from samples from a population-based, double-blind randomized trial of folic acid supplementation (100, 400, 4000 µg per day) taken for 6 months; including a 3 month post-supplementation sample. We observed no changes in global DNA methylation in response to up to 4,000 µg/day for 6 months supplementation in DNA extracted from uncoagulated blood (approximates circulating blood). However, when DNA methylation was determined in coagulated samples from the same individuals at the same time, significant time, dose, and MTHFR genotype-dependent changes were observed. The baseline level of DNA methylation was the same for uncoagulated and coagulated samples; marked differences between sample types were observed only after intervention. In DNA from coagulated blood, DNA methylation decreased (-14%; P<0.001) after 1 month of supplementation and 3 months after supplement withdrawal, methylation decreased an additional 23% (P<0.001) with significant variation among individuals (max+17%; min-94%). Decreases in methylation of ≥25% (vs. <25%) after discontinuation of supplementation were strongly associated with genotype: MTHFR CC vs. TT (adjusted odds ratio [aOR] 12.9, 95%CI 6.4, 26.0). The unexpected difference in DNA methylation between DNA extracted from coagulated and uncoagulated samples in response to folic acid supplementation is an important finding for evaluating use of folic acid and investigating the potential effects of folic acid supplementation on coagulation.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.