ArticleFrontiers in neuroengineering2011
Formation of Essential Ultrastructural Interface between Cultured Hippocampal Cells and Gold Mushroom-Shaped MEA- Toward "IN-CELL" Recordings from Vertebrate Neurons.
Article in Frontiers in neuroengineering, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
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- Tentacle Microelectrode Arrays Uncover Soft Boundary Neurons in Hippocampal CA1.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Architecture design and advanced manufacturing of heart-on-a-chip: scaffolds, stimulation and sensors.Microsystems & nanoengineering · 2024Review
- Graphene nanostructures for input-output bioelectronics.Biophysics reviews · 2021Review
- Tutorial: using nanoneedles for intracellular delivery.Nature protocols · 2021Review
- Intrinsically Conductive Polymers for Striated Cardiac Muscle Repair.International journal of molecular sciences · 2021Review
- High Aspect Ratio and Light-Sensitive Micropillars Based on a Semiconducting Polymer Optically Regulate Neuronal Growth.ACS applied materials & interfaces · 2021Article
- Ultrastructural Analysis of Neuroimplant-Parenchyma Interfaces Uncover Remarkable Neuroregeneration Along-With Barriers That Limit the Implant Electrophysiological Functions.Frontiers in neuroscience · 2021Article
- Recent advances in bioelectronics chemistry.Chemical Society reviews · 2020Review
- Subretinally injected semiconducting polymer nanoparticles rescue vision in a rat model of retinal dystrophy.Nature nanotechnology · 2020Article
- High-Aspect-Ratio Nanostructured Surfaces as Biological Metamaterials.Advanced materials (Deerfield Beach, Fla.) · 2020Review
- Low-Impedance 3D PEDOT:PSS Ultramicroelectrodes.Frontiers in neuroscience · 2020Article
- Interfacing Cells with Vertical Nanoscale Devices: Applications and Characterization.Annual review of analytical chemistry (Palo Alto, Calif.) · 2018Review
- Overexpression of cypin alters dendrite morphology, single neuron activity, and network properties via distinct mechanisms.Journal of neural engineering · 2018Article
- Ultrasensitive gold micro-structured electrodes enabling the detection of extra-cellular long-lasting potentials in astrocytes populations.Scientific reports · 2017Article
- Revealing the Cell-Material Interface with Nanometer Resolution by Focused Ion Beam/Scanning Electron Microscopy.ACS nano · 2017Article
- Next-generation probes, particles, and proteins for neural interfacing.Science advances · 2017Review
- High Density Individually Addressable Nanowire Arrays Record Intracellular Activity from Primary Rodent and Human Stem Cell Derived Neurons.Nano letters · 2017Article
- On-chip, multisite extracellular and intracellular recordings from primary cultured skeletal myotubes.Scientific reports · 2016Article
- Multisite electrophysiological recordings by self-assembled loose-patch-like junctions between cultured hippocampal neurons and mushroom-shaped microelectrodes.Scientific reports · 2016Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using cultured Aplysia neurons we recently reported on the development of a novel approach in which an extracellular, non-invasive multi-electrode-array system provides multisite, attenuated, intracellular recordings of subthreshold synaptic potentials, and action potentials (APs), the so called "IN-CELL" recording configuration (to differentiate it from intracellular recordings). Because of its non-invasive nature, the configuration can be used for long term semi intracellular electrophysiological monitoring of APs and synaptic potentials. Three principals converge to generate the IN-CELL configuration: (a) engulfment of approximately 1 μm size gold mushroom-shaped microelectrodes (gMμE) by the neurons, (b) formation of high seal resistance between the cell's plasma membrane and the engulfed gMμE, and (c), autonomous localized increased conductance of the membrane patch facing the gMμE. Using dissociated rat hippocampal cultures we report here that the necessary morphological and ultrastructural relationships to generate the IN-CELL recording configuration are formed between hippocampal cells and the gMμEs. Interestingly, even <1 μm thin branches expand and engulf the gMμE structures. Recordings of spontaneous electrical activity revealed fast ∼2 ms, 0.04-0.75 mV positive monophasic APs (FPMP). We propose that the FPMP are attenuated APs generated by neurons that engulf gMμEs. Computer simulations of analog electrical circuits depicting the cell-gMμE configuration point out the parameters that should be altered to improve the neuron-gMμE electrical coupling.
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