Evidence map›Paper›PMID 22163185›Full record

ArticleMarine drugs2011

Tasco(®), a product of Ascophyllum nodosum, imparts thermal stress tolerance in Caenorhabditis elegans.

Saveetha Kandasamy, Di Fan, Jatinder Singh Sangha, Wajahatullah Khan, Franklin Evans, Alan T Critchley, Balakrishnan Prithiviraj

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Marine drugs, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Saveetha KandasamyDepartment of Environmental Sciences, Nova Scotia Agricultural College, P.O. Box 550, Truro NS B2B 5E3, Canada.
Di FanDepartment of Environmental Sciences, Nova Scotia Agricultural College, P.O. Box 550, Truro NS B2B 5E3, Canada.
Jatinder Singh SanghaDepartment of Environmental Sciences, Nova Scotia Agricultural College, P.O. Box 550, Truro NS B2B 5E3, Canada.
Wajahatullah KhanGenome Research Chair Unit, Department of Biochemistry, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia.
Franklin EvansAcadian Seaplants Limited, 30 Brown Avenue, Dartmouth NS B3B 1X8, Canada.
Alan T CritchleyAcadian Seaplants Limited, 30 Brown Avenue, Dartmouth NS B3B 1X8, Canada.
Balakrishnan PrithivirajDepartment of Environmental Sciences, Nova Scotia Agricultural College, P.O. Box 550, Truro NS B2B 5E3, Canada.
Nova Scotia Department of Agriculture · CAAcadian Seaplants (Canada) · CAKing Saud University · SA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tasco(®), a commercial product manufactured from the brown alga Ascophyllum nodosum, has been shown to impart thermal stress tolerance in animals. We investigated the physiological, biochemical and molecular bases of this induced thermal stress tolerance using the invertebrate animal model, Caenorhabiditis elegans. Tasco(®) water extract (TWE) at 300 μg/mL significantly enhanced thermal stress tolerance as well as extended the life span of C. elegans. The mean survival rate of the model animals under thermal stress (35 °C) treated with 300 μg/mL and 600 μg/mL TWE, respectively, was 68% and 71% higher than the control animals. However, the TWE treatments did not affect the nematode body length, fertility or the cellular localization of daf-16. On the contrary, TWE under thermal stress significantly increased the pharyngeal pumping rate in treated animals compared to the control. Treatment with TWE also showed differential protein expression profiles over control following 2D gel-electrophoresis analysis. Furthermore, TWE significantly altered the expression of at least 40 proteins under thermal stress; among these proteins 34 were up-regulated while six were down-regulated. Mass spectroscopy analysis of the proteins altered by TWE treatment revealed that these proteins were related to heat stress tolerance, energy metabolism and a muscle structure related protein. Among them heat shock proteins, superoxide dismutase, glutathione peroxidase, aldehyde dehydrogenase, saposin-like proteins 20, myosin regulatory light chain 1, cytochrome c oxidase RAS-like, GTP-binding protein RHO A, OS were significantly up-regulated, while eukaryotic translation initiation factor 5A-1 OS, 60S ribosomal protein L18 OS, peroxiredoxin protein 2 were down regulated by TWE treatment. These results were further validated by gene expression and reporter gene expression analyses. Overall results indicate that the water soluble components of Tasco(®) imparted thermal stress tolerance in the C. elegans by altering stress related biochemical pathways.

Indexed as

Down-RegulationUp-RegulationAnimalsAscophyllumCaenorhabditis elegansDisease Models, AnimalDose-Response Relationship, DrugElectrophoresis, Gel, Two-DimensionalGene Expression ProfilingGenes, ReporterHeat Stress DisordersMass SpectrometryPharynxProteinsSolubilitySurvival RateProteinsAscophyllum nodosumCaenorhabiditis elegansgene expressionlongevityproteomicsstressTasco®thermo tolerance

Identifiers

PMID22163185
PMCPMC3229234
OpenAlexW2160438806

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.