ArticleInternational journal of nanomedicine2011
A rapid and highly sensitive protocol for the detection of Escherichia coli O157:H7 based on immunochromatography assay combined with the enrichment technique of immunomagnetic nanoparticles.
Article in International journal of nanomedicine, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- An Enhanced Lateral Flow Assay Based on Aptamer-Magnetic Separation and Multifold AuNPs for Ultrasensitive Detection ofFoods (Basel, Switzerland) · 2021Article
- Highly sensitive protein detection using recombinant spores and lateral flow immunoassay.Analytical and bioanalytical chemistry · 2021Article
- EDTA-KInternational journal of nanomedicine · 2021Article
- Diagnostic value of platelet-derived microparticles in pulmonary thromboembolism: A population-based study.Experimental and therapeutic medicine · 2018Article
- Nanozyme-Mediated Dual Immunoassay Integrated with Smartphone for Use in Simultaneous Detection of Pathogens.ACS applied materials & interfaces · 2017Article
- Development of multiple cross displacement amplification label-based gold nanoparticles lateral flow biosensor for detection ofInternational journal of nanomedicine · 2017Article
- A review on detection methods used for foodborne pathogens.The Indian journal of medical research · 2016Review
- Sensitive detection of Escherichia coli O157:H7 using Pt-Au bimetal nanoparticles with peroxidase-like amplification.Biosensors & bioelectronics · 2016Article
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Authors and funding
8 authors.
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Abstract
backgroundEscherichia coli O157:H7 (E. coli O157:H7) is an important pathogenic bacterium that threatens human health. A rapid, simple, highly sensitive, and specific method for the detection of E. coli O157:H7 is necessary.
methodsIn the present study, immunomagnetic nanoparticles (IMPs) were prepared with nanopure iron as the core, coated with E. coli O157:H7 polyclonal antibodies. These IMPs were used in combination with immunochromatographic assay (ICA) and used to establish highly sensitive and rapid kits (IMPs+ICA) to detect E. coli O157:H7. The kits were then used to detect E. coli O157:H7 in 150 food samples and were compared with conventional ICA to evaluate their efficacy.
resultsThe average diameter of IMPs was 56 nm and the amount of adsorbed antibodies was 106.0 μg/mg. The sensitivity of ICA and IMPs+ICA was 10(5) colony-forming units/mL and 10(3) CFUs/mL, respectively, for purified E. coli O157:H7 solution. The sensitivity of IMPs+ICA was increased by two orders, and its specificity was similar to ICA.
conclusionThe kits have the potential to offer important social and economic benefits in the screening, monitoring, and control of food safety.
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