ArticleAmerican journal of respiratory cell and molecular biology2012
Albuterol modulates its own transepithelial flux via changes in paracellular permeability.
Article in American journal of respiratory cell and molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Effect of airway acidosis and alkalosis on airway vascular smooth muscle responsiveness to albuterol.BMC pharmacology & toxicology · 2015Trial
- Translational Physiologically Based Pharmacokinetic Modeling to Predict Human Pulmonary Kinetics After Lung Delivery.CPT: pharmacometrics & systems pharmacology · 2025Article
- A 3D Model of the Human Lung Airway for Evaluating Permeability of Inhaled Drugs.ACS pharmacology & translational science · 2025Article
- The Effects of Obesity on Lung Physiology, the Prevalence and Severity of Chronic Pulmonary Diseases, and Inhalation Treatment.Drug design, development and therapy · 2025Review
- Article
- Asthma and COPD: A Focus on β-Agonists - Past, Present and Future.Handbook of experimental pharmacology · 2024Review
- Gene-specific MicroRNA antagonism protects against HIV Tat and TGF-β-mediated suppression of CFTR mRNA and function.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2021Article
- Organic Cation Transporters in the Lung-Current and Emerging (Patho)Physiological and Pharmacological Concepts.International journal of molecular sciences · 2020Review
- The leaky lung test: a pilot study using inhaled mannitol to measure airway barrier function in asthma.The Journal of asthma : official journal of the Association for the Care of Asthma · 2019Article
- TGF-β1 increases viral burden and promotes HIV-1 latency in primary differentiated human bronchial epithelial cells.Scientific reports · 2019Article
- A Neutralizing Aptamer to TGFBR2 and miR-145 Antagonism Rescue Cigarette Smoke- and TGF-β-Mediated CFTR Expression.Molecular therapy : the journal of the American Society of Gene Therapy · 2019Article
- Beta-2 Adrenergic and Glucocorticoid Receptor Agonists Modulate Ozone-Induced Pulmonary Protein Leakage and Inflammation in Healthy and Adrenalectomized Rats.Toxicological sciences : an official journal of the Society of Toxicology · 2018Article
- Cigarette smoke promotes HIV infection of primary bronchial epithelium and additively suppresses CFTR function.Scientific reports · 2018Article
- β₂-Adrenoceptor Blockade Deteriorates Systemic Anaphylaxis by Enhancing Hyperpermeability in Anesthetized Mice.Allergy, asthma & immunology research · 2018Article
- HIV Infects Bronchial Epithelium and Suppresses Components of the Mucociliary Clearance Apparatus.PloS one · 2017Article
- Soluble adenylyl cyclase mediates hydrogen peroxide-induced changes in epithelial barrier function.Respiratory research · 2016Article
- Carvedilol binding to β2-adrenergic receptors inhibits CFTR-dependent anion secretion in airway epithelial cells.American journal of physiology. Lung cellular and molecular physiology · 2016Article
- Transforming growth factor-β1 and cigarette smoke inhibit the ability of β2-agonists to enhance epithelial permeability.American journal of respiratory cell and molecular biology · 2015Article
- Mucociliary dysfunction in HIV and smoked substance abuse.Frontiers in microbiology · 2015Review
- Regulation of β-adrenergic receptor trafficking and lung microvascular endothelial cell permeability by Rab5 GTPase.International journal of biological sciences · 2015Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Although inhaled bronchodilators are commonly used in the treatment of airway disease to dilate airway smooth muscle, little is known regarding the mechanisms that regulate albuterol movement across the epithelium to reach its target, the airway smooth muscle. Because the rate of onset depends on the transepithelial transport of albuterol, to determine the mechanisms that regulate the transepithelial movement of albuterol is essential. Human bronchial epithelial cells, fully redifferentiated in culture at the air-liquid interface, were used to study the cellular uptake and total transepithelial flux of (3)H-albuterol from the apical to the basolateral surfaces. (3)H-mannitol and transepithelial electrical resistance were used to quantify changes in paracellular permeability. The majority of albuterol flux across the epithelium occurred via the paracellular route. The cellular uptake of albuterol was found to be saturable, whereas transepithelial flux was not. Cellular uptake could be inhibited by the amino acids lysine and histidine, with no effect on net transepithelial flux. Transepithelial flux was altered by maneuvers that collapsed or disrupted intercellular junctions. Acidification, usually seen in exacerbations of airway disease, decreased albuterol flux. In addition, albuterol increased its own paracellular permeability. The ability of albuterol to modulate paracellular permeability was blocked by the β(2)-adrenergic receptor-selective antagonist ICI 118551. Albuterol mainly crosses the epithelium via the paracellular pathway, but has the ability to modulate its own permeability through changes in the leakiness of tight junctions, which is modulated through the signaling of the β(2)-adrenergic receptor.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.