ArticleJournal of experimental botany2012
Identification, functional characterization, and regulation of the enzyme responsible for floral (E)-nerolidol biosynthesis in kiwifruit (Actinidia chinensis).
Article in Journal of experimental botany, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 87 citations in OpenAlex.
- From monthly volatile dynamics to cultivar differences in the aroma of jasmine (Food chemistry: X · 2026Article
- When cold adjusts volatile metabolism: CBF4 and WRKY4 activator-repressor module controls nerolidol glucosylation in tea plants.Plant physiology · 2026Article
- Review
- Enabling and improvingFrontiers in bioengineering and biotechnology · 2025Article
- Aphid alarm pheromone mimicry in transgenicFrontiers in plant science · 2024Article
- Product Profiles of Promiscuous Enzymes Can be Altered by Controlling In Vivo Spatial Organization.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- The terpene synthase (TPS) gene family in kiwifruit shows high functional redundancy and a subset of TPS likely fulfil overlapping functions in fruit flavour, floral bouquet and defence.Molecular horticulture · 2023Article
- Cis-Nerolidol Inhibits MAP Kinase and NF-κB Signaling Pathways and Prevents Epithelial Tight Junction Dysfunction in Colon Inflammation: In Vivo and In Vitro Studies.Molecules (Basel, Switzerland) · 2023Article
- Neutrophil Immunomodulatory Activity of Nerolidol, a Major Component of Essential Oils fromPlants (Basel, Switzerland) · 2022Article
- Comparative methylomics and chromatin accessibility analysis inHorticulture research · 2022Article
- Chromosome-level genome assembly and resequencing of camphor tree (Horticulture research · 2022Article
- Kinetic studies and homology modeling of a dual-substrate linalool/nerolidol synthase from Plectranthus amboinicus.Scientific reports · 2021Article
- Nerolidol Attenuates Oxidative Stress, Inflammation, and Apoptosis by Modulating Nrf2/MAPK Signaling Pathways in Doxorubicin-Induced Acute Cardiotoxicity in Rats.Antioxidants (Basel, Switzerland) · 2021Article
- Nerolidol Mitigates Colonic Inflammation: An Experimental Study Using both In Vivo and In Vitro Models.Nutrients · 2020Article
- Molecular Cloning and Functional Characterization ofPlants (Basel, Switzerland) · 2020Article
- Sensory-Directed Genetic and Biochemical Characterization of Volatile Terpene Production in Kiwifruit.Plant physiology · 2020Article
- Analysis of Terpene Synthase Family Genes in Camellia sinensis with an Emphasis on Abiotic Stress Conditions.Scientific reports · 2020Article
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- Identification and characterization of terpene synthase genes accounting for volatile terpene emissions in flowers of Freesia x hybrida.Journal of experimental botany · 2018Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flowers of the kiwifruit species Actinidia chinensis produce a mixture of sesquiterpenes derived from farnesyl diphosphate (FDP) and monoterpenes derived from geranyl diphosphate (GDP). The tertiary sesquiterpene alcohol (E)-nerolidol was the major emitted volatile detected by headspace analysis. Contrastingly, in solvent extracts of the flowers, unusually high amounts of (E,E)-farnesol were observed, as well as lesser amounts of (E)-nerolidol, various farnesol and farnesal isomers, and linalool. Using a genomics-based approach, a single gene (AcNES1) was identified in an A. chinensis expressed sequence tag library that had significant homology to known floral terpene synthase enzymes. In vitro characterization of recombinant AcNES1 revealed it was an enzyme that could catalyse the conversion of FDP and GDP to the respective (E)-nerolidol and linalool terpene alcohols. Enantiomeric analysis of both AcNES1 products in vitro and floral terpenes in planta showed that (S)-(E)-nerolidol was the predominant enantiomer. Real-time PCR analysis indicated peak expression of AcNES1 correlated with peak (E)-nerolidol, but not linalool accumulation in flowers. This result, together with subcellular protein localization to the cytoplasm, indicated that AcNES1 was acting as a (S)-(E)-nerolidol synthase in A. chinensis flowers. The synthesis of high (E,E)-farnesol levels appears to compete for the available pool of FDP utilized by AcNES1 for sesquiterpene biosynthesis and hence strongly influences the accumulation and emission of (E)-nerolidol in A. chinensis flowers.
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