ArticlePloS one2011
Substrate adhesion regulates sealing zone architecture and dynamics in cultured osteoclasts.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Aesculetin Inhibits Osteoclastic Bone Resorption through Blocking Ruffled Border Formation and Lysosomal Trafficking.International journal of molecular sciences · 2020Article
- Myosins in Osteoclast Formation and Function.Biomolecules · 2018Review
- Intravital microscopy of osteolytic progression and therapy response of cancer lesions in the bone.Science translational medicine · 2018Article
- Article
- Dynamics of the sealing zone in cultured osteoclasts.Cytoskeleton (Hoboken, N.J.) · 2017Article
- Surface microtopography modulates sealing zone development in osteoclasts cultured on bone.Journal of the Royal Society, Interface · 2017Article
- In vitro model of bone to facilitate measurement of adhesion forces and super-resolution imaging of osteoclasts.Scientific reports · 2016Article
- Podosomes in space: macrophage migration and matrix degradation in 2D and 3D settings.Cell adhesion & migration · 2014Review
- The RhoGAP activity of myosin IXB is critical for osteoclast podosome patterning, motility, and resorptive capacity.PloS one · 2014Article
- Multifunctional role of osteopontin in directing intrafibrillar mineralization of collagen and activation of osteoclasts.Acta biomaterialia · 2014Article
- Osteoclasts on bone and dentin in vitro: mechanism of trail formation and comparison of resorption behavior.Calcified tissue international · 2013Article
- Precursors linked via the zipper-like structure or the filopodium during the secondary fusion of osteoclasts.Communicative & integrative biology · 2012Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bone-degrading activity of osteoclasts depends on the formation of a cytoskeletal-adhesive super-structure known as the sealing zone (SZ). The SZ is a dynamic structure, consisting of a condensed array of podosomes, the elementary adhesion-mediating structures of osteoclasts, interconnected by F-actin filaments. The molecular composition and structure of the SZ were extensively investigated, yet despite its major importance for bone formation and remodelling, the mechanisms underlying its assembly and dynamics are still poorly understood. Here we determine the relations between matrix adhesiveness and the formation, stability and expansion of the SZ. By growing differentiated osteoclasts on micro-patterned glass substrates, where adhesive areas are separated by non-adhesive PLL-g-PEG barriers, we show that SZ growth and fusion strictly depend on the continuity of substrate adhesiveness, at the micrometer scale. We present a possible model for the role of mechanical forces in SZ formation and reorganization, inspired by the current data.
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