ArticleProceedings of the National Academy of Sciences of the United States of America2012
Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 127 papers.
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Who cites it
127 citing papers in PubMed.
- A Versatile Tool to Predict and Guide RESOLFT Images Based on Photoswitching, Labelling and Optical Properties.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- Multimodal Super-Resolution Imaging of Nitrogen-Vacancy Centers via High-Index-Induced Structured Illumination Microscopy and Optically Detected Magnetic Resonance Spectrometry.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- All-optical strategies to minimize photobleaching in reversibly switchable fluorescent proteins.Nature communications · 2025Article
- Perspective: An outlook on fluorescence tracking.The Journal of chemical physics · 2025Article
- Two-dimensional nonlinear structured illumination microscopy with rsEGFP2.Biomedical optics express · 2025Article
- Photoswitchable Fluorescent Hydrazone for Super-Resolution Cell Membrane Imaging.Journal of the American Chemical Society · 2025Article
- SOGO-SOFI, light-modulated super-resolution optical fluctuation imaging using only 20 raw frames for high-fidelity reconstruction.Fundamental research · 2025Article
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- Super-resolution imaging of fast morphological dynamics of neurons in behaving animals.Nature methods · 2025Article
- Exploring Transient States of PAmKate to Enable Improved Cryogenic Single-Molecule Imaging.Journal of the American Chemical Society · 2024Article
- Insight into Single-Molecule Imaging Techniques for the Study of Prokaryotic Genome Maintenance.Chemical & biomedical imaging · 2024Review
- Lanthanide ion-doped upconversion nanoparticles for low-energy super-resolution applications.Light, science & applications · 2024Review
- Exploring transient states of PAmKate to enable improved cryogenic single-molecule imaging.bioRxiv : the preprint server for biology · 2024Article
- Probing the dynamic crosstalk of lysosomes and mitochondria with structured illumination microscopy.Trends in analytical chemistry : TRAC · 2023Article
- Article
- Advanced imaging techniques for tracking drug dynamics at the subcellular level.Advanced drug delivery reviews · 2023Review
- Superresolution structured illumination microscopy reconstruction algorithms: a review.Light, science & applications · 2023Review
- Far-field super-resolution chemical microscopy.Light, science & applications · 2023Review
- Small training dataset convolutional neural networks for application-specific super-resolution microscopy.Journal of biomedical optics · 2023Article
- Transmission structured illumination microscopy with tunable frequency illumination using tilt mirror assembly.Scientific reports · 2023Article
67 more citing papers are in PubMed but not listed here.
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8 authors.
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Abstract
Using ultralow light intensities that are well suited for investigating biological samples, we demonstrate whole-cell superresolution imaging by nonlinear structured-illumination microscopy. Structured-illumination microscopy can increase the spatial resolution of a wide-field light microscope by a factor of two, with greater resolution extension possible if the emission rate of the sample responds nonlinearly to the illumination intensity. Saturating the fluorophore excited state is one such nonlinear response, and a realization of this idea, saturated structured-illumination microscopy, has achieved approximately 50-nm resolution on dye-filled polystyrene beads. Unfortunately, because saturation requires extremely high light intensities that are likely to accelerate photobleaching and damage even fixed tissue, this implementation is of limited use for studying biological samples. Here, reversible photoswitching of a fluorescent protein provides the required nonlinearity at light intensities six orders of magnitude lower than those needed for saturation. We experimentally demonstrate approximately 40-nm resolution on purified microtubules labeled with the fluorescent photoswitchable protein Dronpa, and we visualize cellular structures by imaging the mammalian nuclear pore and actin cytoskeleton. As a result, nonlinear structured-illumination microscopy is now a biologically compatible superresolution imaging method.
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