ArticleClinical and experimental nephrology2012
Daily variance of urinary excretion of AQP2 determined by sandwich ELISA method.
Article in Clinical and experimental nephrology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Hyponatremia Associated with Congestive Heart Failure: Involvement of Vasopressin and Efficacy of Vasopressin Receptor Antagonists.Journal of clinical medicine · 2023Article
- Updates and Perspectives on Aquaporin-2 and Water Balance Disorders.International journal of molecular sciences · 2021Review
- Urinary Aquaporin 2 as a Potential Indicator Predicting Tolvaptan Response in Patients With ADPKD.Kidney international reports · 2021Article
- Perioperative urinary excretion of aquaporin-2 dependent upon vasopressin in cardiac surgery.Heart and vessels · 2020Observational
- AQP2 in human urine is predominantly localized to exosomes with preserved water channel activities.Clinical and experimental nephrology · 2018Article
- Circadian regulation of renal function.Free radical biology & medicine · 2018Review
- Is Exaggerated Release of Arginine Vasopressin an Endocrine Disorder? Pathophysiology and Treatment.Journal of clinical medicine · 2017Review
- Disruption of Membranes of Extracellular Vesicles Is Necessary for ELISA Determination of Urine AQP2: Proof of Disruption and Epitopes of AQP2 Antibodies.International journal of molecular sciences · 2016Article
- Urinary excretion of the water channel aquaporin 2 correlated with the pharmacological effect of tolvaptan in cirrhotic patients with ascites.Journal of gastroenterology · 2016Article
- Alkali treatment stabilizes fluctuations of urine AQP2 values measured by ELISA.Clinical and experimental nephrology · 2016Article
- Urine Aquaporin-2: A Promising Marker of Response to the Arginine Vasopressin Type-2 Antagonist, Tolvaptan in Patients with Congestive Heart Failure.International journal of molecular sciences · 2016Review
- Dynamic regulation and dysregulation of the water channel aquaporin-2: a common cause of and promising therapeutic target for water balance disorders.Clinical and experimental nephrology · 2014Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundUrinary excretion of aquaporin 2 (AQP2) is a useful marker of kidney collecting duct function. A specific and convenient method to measure AQP2 in human urine would help to treat water balance disorders. It is unknown whether urinary excretion of AQP2 shows any daily variance.
methodsA sandwich enzyme-linked immunosorbent assay (ELISA) method for AQP2 was established using two different kinds of antibodies, and its sensitivity and specificity were examined. Daily variance of urinary excretion of AQP2 and responses to acute water load were examined.
resultsThe established ELISA specifically detected urine AQP2 with high sensitivity (detected as low as 0.34 pmol/mL). Urinary excretion of AQP2 did not show daily variance between 9 a.m. and 9 p.m. in healthy subjects.
conclusionsThe developed ELISA method using two different antibodies is convenient and highly sensitive, and could be useful in clinical practice. Urinary excretion of AQP2 is relatively constant from morning to night, and spot urine sampling at any time during this time period does not affect the results.
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