ArticleMolecular biology of the cell2012
Calpains promote α2β1 integrin turnover in nonrecycling integrin pathway.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Enhanced Tumor-Targeted Delivery of Arginine-Rich Peptides via a Positive Feedback Loop Orchestrated by Piezo1/integrin β1 Signaling Axis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Covalent and non-covalent coupling of a AuNanoscale advances · 2021Article
- Calcium-Dependent Calpain Activation-Mediated Mitochondrial Dysfunction and Oxidative Stress Are Required for Cytotoxicity of Epinecidin-1 in Human Synovial Sarcoma SW982 Cells.International journal of molecular sciences · 2020Article
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- Slow Infection due to Lowering the Amount of Intact versus Empty Particles Is a Characteristic Feature of Coxsackievirus B5 Dictated by the Structural Proteins.Journal of virology · 2019Article
- Calpain2 mediates Rab5-driven focal adhesion disassembly and cell migration.Cell adhesion & migration · 2018Article
- Epstein-Barr virus-encoded LMP2A stimulates migration of nasopharyngeal carcinoma cells via the EGFR/CaBiology open · 2017Article
- Distinct activity of the bone-targeted gallium compound KP46 against osteosarcoma cells - synergism with autophagy inhibition.Journal of experimental & clinical cancer research : CR · 2017Article
- Infectious Entry Pathway of Enterovirus B Species.Viruses · 2015Review
- The multivesicular body is the major internal site of prion conversion.Journal of cell science · 2015Article
- Coxsackievirus A9 infects cells via nonacidic multivesicular bodies.Journal of virology · 2014Article
- Permeability changes of integrin-containing multivesicular structures triggered by picornavirus entry.PloS one · 2014Article
- Cholesterol dependence of collagen and echovirus 1 trafficking along the novel α2β1 integrin internalization pathway.PloS one · 2013Article
- Article
- Endocytosis of integrin-binding human picornaviruses.Advances in virology · 2012Article
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 2 countries.
Funding
Abstract
Collagen receptor integrins recycle between the plasma membrane and endosomes and facilitate formation and turnover of focal adhesions. In contrast, clustering of α2β1 integrin with antibodies or the human pathogen echovirus 1 (EV1) causes redistribution of α2 integrin to perinuclear multivesicular bodies, α2-MVBs. We show here that the internalized clustered α2 integrin remains in α2-MVBs and is not recycled back to the plasma membrane. Instead, receptor clustering and internalization lead to an accelerated down-regulation of α2β1 integrin compared to the slow turnover of unclustered α2 integrin. EV1 infection or integrin degradation is not associated with proteasomal or autophagosomal processes and shows no significant association with lysosomal pathway. In contrast, degradation is dependent on calpains, such that it is blocked by calpain inhibitors. We show that active calpain is present in α2-MVBs, internalized clustered α2β1 integrin coprecipitates with calpain-1, and calpain enzymes can degrade α2β1 integrin. In conclusion, we identified a novel virus- and clustering-specific pathway that diverts α2β1 integrin from its normal endo/exocytic traffic to a nonrecycling, calpain-dependent degradative endosomal route.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.