ArticleMolecular biology reports2012
Characterization and expression of attacin, an antibacterial protein-encoding gene, from the beet armyworm, Spodoptera exigua (Hübner) (Insecta: Lepidoptera: Noctuidae).
Article in Molecular biology reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 53 citations in OpenAlex.
- Calcineurin-dependent 20E signaling pathway enhances attacin expression to prime the immunity of Helicoverpa armigera against Bacillus thuringiensis Cry1Ac toxin.PLoS pathogens · 2026Article
- Mechanism, Efficacy, and Safety of Natural Antibiotics.Antibiotics (Basel, Switzerland) · 2025Review
- Identification and antibacterial activity of a novel antimicrobial peptide attacin from Conogethes punctiferalis.Archives of microbiology · 2025Article
- Article
- Insect Antimicrobial Peptides as Guardians of Immunity and Beyond: A Review.International journal of molecular sciences · 2024Review
- Heterologous Expression and Bioactivity Determination ofInternational journal of molecular sciences · 2023Article
- Insect-pathogen crosstalk and the cellular-molecular mechanisms of insect immunity: uncovering the underlying signaling pathways and immune regulatory function of non-coding RNAs.Frontiers in immunology · 2023Review
- The effect of combined knockdowns of Attacins on survival and bacterial load inFrontiers in immunology · 2023Article
- Evaluation of antibacterial activity induced bySaudi journal of biological sciences · 2022Article
- Concerted impacts of antiherbivore defenses and opportunistic Serratia pathogens on the fall armyworm (Spodoptera frugiperda).Oecologia · 2022Article
- Insect antimicrobial peptides: potential weapons to counteract the antibiotic resistance.Cellular and molecular life sciences : CMLS · 2021Review
- Immune-related genes of the larval Holotrichia parallela in response to entomopathogenic nematodes Heterorhabditis beicherriana LF.BMC genomics · 2021Article
- Attacins: A Promising Class of Insect Antimicrobial Peptides.Antibiotics (Basel, Switzerland) · 2021Review
- Article
- Antimicrobial Peptides: Novel Source and Biological Function With a Special Focus on Entomopathogenic Nematode/Bacterium Symbiotic Complex.Frontiers in microbiology · 2021Review
- iTRAQ-Based Comparative Proteomic Analysis of Larval Midgut From the Beet Armyworm,Frontiers in physiology · 2020Article
- Hemocyte-hemocyte adhesion by granulocytes is associated with cellular immunity in the cricket, Gryllus bimaculatus.Scientific reports · 2019Article
- Review
- Fusion proteins towards fungi and bacteria in plant protection.Microbiology (Reading, England) · 2018Article
- An antimicrobial peptide-resistant minor subpopulation of Photorhabdus luminescens is responsible for virulence.Scientific reports · 2017Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To isolate antimicrobial-related genes from the beet armyworm, Spodoptera exigua, we performed GeneFishing, a polymerase chain reaction (PCR)-based differential display technique. An attacin-like complementary DNA (cDNA) including a 3'-untranslated region was identified from among 18 over-expressed genes in microbial-infected larvae. The full-length attacin cDNA from S. exigua cDNA (Seattacin) was cloned using rapid amplification of cDNA ends PCR. The attacin-like cDNA transcript was 765 nucleotides in length, and the predicted polypeptide was 254 amino acids in length with a calculated molecular mass of 27.6 kDa and an isoelectric point of 6.44. The protein sequence of the attacin-like cDNA showed high identity to that of Trichoplusia ni (61.2%). The amino acid sequence identity of Seattacin to the orthologous proteins in Bombyx mori, Manduca sexta, Heliothis virescens, Hlicoverpa armigera, Hyphantria cunea, Hyalophora cecropia, and Drosophila melanogaster was 61.2, 46.1, 44.5, 42.2, 39.5, 45.1, and 24.0%, respectively. To examine possible immune functions of the attacin-like cDNA, its expression was investigated by reverse transcriptase PCR analysis after challenging S. exigua with microorganisms. The attacin-like cDNA was expressed at high levels 12 h post-infection, and its expression was slightly induced 4-8 h post-infection compared to control larvae inoculated with sterile water. Furthermore, induced Seattacin showed biological activity against several bacteria including Escherichia coli DH5α, Pseudomonas cichorii, Bacillus subtilis, and Listeria monocytogenes. These results suggest that the attacin-like cDNA of S. exigua codes for antimicrobial peptides.
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