ReviewJournal of industrial microbiology & biotechnology2012
Streptomyces temperate bacteriophage integration systems for stable genetic engineering of actinomycetes (and other organisms).
Review in Journal of industrial microbiology & biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 88 citations in OpenAlex.
- A new-engineered integrative tool to target the terminal compartment of the Streptomyces chromosome.Applied microbiology and biotechnology · 2026Article
- Fluorescence-Based Soil Survival Analysis of the Xenobiotic- and Metal-DetoxifyingInternational journal of molecular sciences · 2025Article
- dTSR enables efficient improvement of heterologous production of spinosad inSynthetic and systems biotechnology · 2025Article
- Enhancement of Acarbose Production inJournal of agricultural and food chemistry · 2025Article
- Mitigating genetic instability caused by the excision activity of theApplied and environmental microbiology · 2025Article
- Current Approaches for Genetic Manipulation ofBiotech (Basel (Switzerland)) · 2025Review
- Thermophilic site-specific recombination system for rapid insertion of heterologous DNA into the Clostridium thermocellum chromosome.Journal of industrial microbiology & biotechnology · 2024Article
- Overexpression of RuBisCO form I and II genes inApplied and environmental microbiology · 2024Article
- High-titer production of staurosporine by heterologous expression and process optimization.Applied microbiology and biotechnology · 2023Article
- Complete genomes and comparative analyses of Streptomyces phages that influence secondary metabolism and sporulation.Scientific reports · 2023Article
- Complete Genome Sequences of Actinobacteriophages Anaysia and Caviar.Microbiology resource announcements · 2022Article
- Genome Sequence of Cluster BI1 Streptomyces griseus Phage TaidaOne.Microbiology resource announcements · 2022Article
- An extended catalogue of ncRNAs in Streptomyces coelicolor reporting abundant tmRNA, RNase-P RNA and RNA fragments derived from pre-ribosomal RNA leader sequences.Archives of microbiology · 2022Article
- Delineation of mechanistic approaches of rhizosphere microorganisms facilitated plant health and resilience under challenging conditions.3 Biotech · 2022Review
- Understanding and Manipulating Assembly Line Biosynthesis by Heterologous Expression in Streptomyces.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Microbial production of small peptide: pathway engineering and synthetic biology.Microbial biotechnology · 2021Review
- Characterization and engineering of Streptomyces griseofuscus DSM 40191 as a potential host for heterologous expression of biosynthetic gene clusters.Scientific reports · 2021Article
- Review
- Approaches to genetic tool development for rapid domestication of non-model microorganisms.Biotechnology for biofuels · 2021Review
- Review
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ϕC31, ϕBT1, R4, and TG1 are temperate bacteriophages with broad host specificity for species of the genus Streptomyces. They form lysogens by integrating site-specifically into diverse attB sites located within individual structural genes that map to the conserved core region of streptomycete linear chromosomes. The target genes containing the ϕC31, ϕBT1, R4, and TG1 attB sites encode a pirin-like protein, an integral membrane protein, an acyl-CoA synthetase, and an aminotransferase, respectively. These genes are highly conserved within the genus Streptomyces, and somewhat conserved within other actinomycetes. In each case, integration is mediated by a large serine recombinase that catalyzes unidirectional recombination between the bacteriophage attP and chromosomal attB sites. The unidirectional nature of the integration mechanism has been exploited in genetic engineering to produce stable recombinants of streptomycetes, other actinomycetes, eucaryotes, and archaea. The ϕC31 attachment/integration (Att/Int) system has been the most widely used, and it has been coupled with the ϕBT1 Att/Int system to facilitate combinatorial biosynthesis of novel lipopeptide antibiotics in Streptomyces fradiae.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.