ArticleAmerican journal of physiology. Heart and circulatory physiology2012
Prostacyclin receptor-mediated ATP release from erythrocytes requires the voltage-dependent anion channel.
Article in American journal of physiology. Heart and circulatory physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 58 citations in OpenAlex.
- CR1-mediated ATP release by human red blood cells promotes CR1 clustering and modulates the immune transfer process.The Journal of biological chemistry · 2013Trial
- Hypoxia induces robust ATP release from erythrocytes in ApoE-LDLR double-deficient mice.Frontiers in physiology · 2024Article
- The impact of nebulized epoprostenol and iloprost on hemoglobin oxygen affinity: an ex vivo experiment.American journal of physiology. Lung cellular and molecular physiology · 2022Article
- Role of Erythrocytes in Nitric Oxide Metabolism and Paracrine Regulation of Endothelial Function.Antioxidants (Basel, Switzerland) · 2022Review
- Lytic Release of Cellular ATP: Physiological Relevance and Therapeutic Applications.Life (Basel, Switzerland) · 2021Review
- VDAC1 at the Intersection of Cell Metabolism, Apoptosis, and Diseases.Biomolecules · 2020Review
- Alteration of purinergic signaling in diabetes: Focus on vascular function.Journal of molecular and cellular cardiology · 2020Review
- Induction of ATP Release, PPIX Transport, and Cholesterol Uptake by Human Red Blood Cells Using a New Family of TSPO Ligands.International journal of molecular sciences · 2018Article
- Human erythrocytes release ATP by a novel pathway involving VDAC oligomerization independent of pannexin-1.Scientific reports · 2018Article
- Erythrocyte's aging in microgravity highlights how environmental stimuli shape metabolism and morphology.Scientific reports · 2018Article
- Purinergic control of red blood cell metabolism: novel strategies to improve red cell storage quality.Blood transfusion = Trasfusione del sangue · 2017Review
- Mitochondrial biogenesis dysfunction and metabolic dysfunction from a novel mitochondrial tRNAScientific reports · 2017Article
- Effects of Hypoxia on Erythrocyte Membrane Properties-Implications for Intravascular Hemolysis and Purinergic Control of Blood Flow.Frontiers in physiology · 2017Review
- New insights on the regulation of the adenine nucleotide pool of erythrocytes in mouse models.PloS one · 2017Article
- Article
- TSPO ligands stimulate ZnPPIX transport and ROS accumulation leading to the inhibition of P. falciparum growth in human blood.Scientific reports · 2016Article
- Role of erythrocyte-released ATP in the regulation of microvascular oxygen supply in skeletal muscle.Acta physiologica (Oxford, England) · 2016Review
- Red Blood Cell Homeostasis: Pharmacological Interventions to Explore Biochemical, Morphological and Mechanical Properties.Frontiers in molecular biosciences · 2016Article
- Article
- Blood cells: an historical account of the roles of purinergic signalling.Purinergic signalling · 2015Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Erythrocytes have been implicated as controllers of vascular caliber by virtue of their ability to release the vasodilator ATP in response to local physiological and pharmacological stimuli. The regulated release of ATP from erythrocytes requires activation of a signaling pathway involving G proteins (G(i) or G(s)), adenylyl cyclase, protein kinase A, and the cystic fibrosis transmembrane conductance regulator as well as a final conduit through which this highly charged anion exits the cell. Although pannexin 1 has been shown to be the final conduit for ATP release from human erythrocytes in response to reduced oxygen tension, it does not participate in transport of ATP following stimulation of the prostacyclin (IP) receptor in these cells, which suggests that an additional protein must be involved. Using antibodies directed against voltage-dependent anion channel (VDAC)1, we confirm that this protein is present in human erythrocyte membranes. To address the role of VDAC in ATP release, two structurally dissimilar VDAC inhibitors, Bcl-x(L) BH4(4-23) and TRO19622, were used. In response to the IP receptor agonists, iloprost and UT-15C, ATP release was inhibited by both VDAC inhibitors although neither iloprost-induced cAMP accumulation nor total intracellular ATP concentration were altered. Together, these findings support the hypothesis that VDAC is the ATP conduit in the IP receptor-mediated signaling pathway in human erythrocytes. In addition, neither the pannexin inhibitor carbenoxolone nor Bcl-x(L) BH4(4-23) attenuated ATP release in response to incubation of erythrocytes with the β-adrenergic receptor agonist isoproterenol, suggesting the presence of yet another channel for ATP release from human erythrocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.