ArticlePlant cell reports2012
Expression of the major mugwort pollen allergen Art v 1 in tobacco plants and cell cultures: problems and perspectives for allergen production in plants.
Article in Plant cell reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Immunotherapy using algal-produced Ara h 1 core domain suppresses peanut allergy in mice.Plant biotechnology journal · 2016Article
- Review
- Recent advances on host plants and expression cassettes' structure and function in plant molecular pharming.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2014Review
- Plant molecular pharming 2012 and beyond.Plant cell reports · 2012Article
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
An economic and cheap production of large amounts of recombinant allergenic proteins might become a prerequisite for the common use of microarray-based diagnostic allergy assays which allow a component-specific diagnosis. A molecular pharming strategy was applied to express the major allergen of Artemisia vulgaris pollen, Art v 1, in tobacco plants and tobacco cell cultures. The original Art v 1 with its endogenous signal peptide which directs Art v 1 to the secretory pathway, was expressed in transiently transformed tobacco leaves but was lost in stable transformed tobacco plants during the alternation of generations. Using a light-regulated promoter and "hiding" the recombinant Art v 1 in the ER succeeded in expression of Art v 1 over three generations of tobacco plants and in cell cultures generated from stable transformed plants. However, the amounts of the recombinant allergen were sufficient for analysis but not high enough to allow an economic production. Although molecular pharming has been shown to work well for the production of non-plant therapeutic proteins, it might be less efficient for closely related plant proteins.
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