ArticleCalcified tissue international2012
Evaluation of methylation status of the eNOS promoter at birth in relation to childhood bone mineral content.
Article in Calcified tissue international, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.
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- The Role of Epigenomics in Osteoporosis and Osteoporotic Vertebral Fracture.International journal of molecular sciences · 2020Review
- Developmental Programming of Body Composition: Update on Evidence and Mechanisms.Current diabetes reports · 2019Review
- Protocol for the EMPHASIS study; epigenetic mechanisms linking maternal pre-conceptional nutrition and children's health in India and Sub-Saharan Africa.BMC nutrition · 2017Article
- Developmental aspects of a life course approach to healthy ageing.The Journal of physiology · 2016Review
- How to interpret epigenetic association studies: a guide for clinicians.BoneKEy reports · 2016Review
- Epigenetics of Osteoporosis: Critical Analysis of Epigenetic Epidemiology Studies.Current genomics · 2015Article
- Life-course approach to nutrition.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2015Review
- Longitudinal analysis of DNA methylation associated with birth weight and gestational age.Human molecular genetics · 2015Article
- CpG signalling, H2A.Z/H3 acetylation and microRNA-mediated deferred self-attenuation orchestrate foetal NOS3 expression.Clinical epigenetics · 2015Article
- Childhood bone mineral content is associated with methylation status of the RXRA promoter at birth.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2014Article
- Growth from birth to adulthood and bone phenotype in early old age: a British birth cohort study.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2014Article
- Article
- The role of longitudinal cohort studies in epigenetic epidemiology: challenges and opportunities.Genome biology · 2012Article
Corrections and comments
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Authors and funding
16 authors at 5 institutions in 4 countries.
Funding
Abstract
Our previous work has shown associations between childhood adiposity and perinatal methylation status of several genes in umbilical cord tissue, including endothelial nitric oxide synthase (eNOS). There is increasing evidence that eNOS is important in bone metabolism; we therefore related the methylation status of the eNOS gene promoter in stored umbilical cord to childhood bone size and density in a group of 9-year-old children. We used Sequenom MassARRAY to assess the methylation status of two CpGs in the eNOS promoter, identified from our previous study, in stored umbilical cords of 66 children who formed part of a Southampton birth cohort and who had measurements of bone size and density at age 9 years (Lunar DPXL DXA instrument). Percentage methylation varied greatly between subjects. For one of the two CpGs, eNOS chr7:150315553 + , after taking account of age and sex, there were strong positive associations between methylation status and the child's whole-body bone area (r = 0.28, P = 0.02), bone mineral content (r = 0.34, P = 0.005), and areal bone mineral density (r = 0.34, P = 0.005) at age 9 years. These associations were independent of previously documented maternal determinants of offspring bone mass. Our findings suggest an association between methylation status at birth of a specific CpG within the eNOS promoter and bone mineral content in childhood. This supports a role for eNOS in bone growth and metabolism and implies that its contribution may at least in part occur during early skeletal development.
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