ArticleEukaryotic cell2012
Enigmatic presence of mitochondrial complex I in Trypanosoma brucei bloodstream forms.
Article in Eukaryotic cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Conserved and divergent mitochondrial assemblies in kinetoplastid parasites.Molecular cell · 2026Article
- Kinetoplast DNA structure, RNA editing patterns and small respiratory Complex I in Trypanosoma musculi.Communications biology · 2025Article
- Comparative mitochondrial genome and transcriptome analyses reveal strain-specific features of RNA editing in Trypanosoma brucei.Nucleic acids research · 2025Article
- Uncovering the Mechanism of Action of Antiprotozoal Agents: A Survey on Photoaffinity Labeling Strategy.Pharmaceuticals (Basel, Switzerland) · 2024Review
- Blastocrithidia nonstop mitochondrial genome and its expression are remarkably insulated from nuclear codon reassignment.Nucleic acids research · 2024Article
- Mitochondrion of the Trypanosoma brucei long slender bloodstream form is capable of ATP production by substrate-level phosphorylation.PLoS pathogens · 2023Article
- Novel insertions in the mitochondrial maxicircle ofParasitology · 2022Article
- Repurposing of MitoTam: Novel Anti-Cancer Drug Candidate Exhibits Potent Activity against Major Protozoan and Fungal Pathogens.Antimicrobial agents and chemotherapy · 2022Article
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- Mitochondrial CaInternational review of cell and molecular biology · 2021Review
- Repurposing bioenergetic modulators against protozoan parasites responsible for tropical diseases.International journal for parasitology. Drugs and drug resistance · 2020Article
- Developmental regulation of edited CYb and COIII mitochondrial mRNAs is achieved by distinct mechanisms in Trypanosoma brucei.Nucleic acids research · 2020Article
- Cell-line specific RNA editing patterns in Trypanosoma brucei suggest a unique mechanism to generate protein variation in a system intolerant to genetic mutations.Nucleic acids research · 2020Article
- A post-transcriptional respiratome regulon in trypanosomes.Nucleic acids research · 2019Article
- Respiratory chain Complex I of unparalleled divergence in diplonemids.The Journal of biological chemistry · 2018Article
- Mitochondrial DNA is critical for longevity and metabolism of transmission stage Trypanosoma brucei.PLoS pathogens · 2018Article
- A paradigm shift: The mitoproteomes of procyclic and bloodstream Trypanosoma brucei are comparably complex.PLoS pathogens · 2017Review
- Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues.PLoS neglected tropical diseases · 2017Article
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Authors and funding
5 authors.
Funding
Abstract
The presence of mitochondrial respiratory complex I in the pathogenic bloodstream stages of Trypanosoma brucei has been vigorously debated: increased expression of mitochondrially encoded functional complex I mRNAs is countered by low levels of enzymatic activity that show marginal inhibition by the specific inhibitor rotenone. We now show that epitope-tagged versions of multiple complex I subunits assemble into α and β subcomplexes in the bloodstream stage and that these subcomplexes require the mitochondrial genome for their assembly. Despite the presence of these large (740- and 855-kDa) multisubunit complexes, the electron transport activity of complex I is not essential under experimental conditions since null mutants of two core genes (NUBM and NUKM) showed no growth defect in vitro or in mouse infection. Furthermore, the null mutants showed no decrease in NADH:ubiquinone oxidoreductase activity, suggesting that the observed activity is not contributed by complex I. This work conclusively shows that despite the synthesis and assembly of subunit proteins, the enzymatic function of the largest respiratory complex is neither significant nor important in the bloodstream stage. This situation appears to be in striking contrast to that for the other respiratory complexes in this parasite, where physical presence in a life-cycle stage always indicates functional significance.
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