ArticlePlant physiology2012
Regulation of high-affinity nitrate uptake in roots of Arabidopsis depends predominantly on posttranscriptional control of the NRT2.1/NAR2.1 transport system.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 99 citations in OpenAlex.
- SaNAR2.2, A Novel Member of the NAR2 Family from the EuhalophyteMembranes · 2026Article
- High-throughput screening for biostimulant discovery: opportunities to enhance nitrogen use efficiency.Frontiers in plant science · 2026Review
- Establishment and maintenance of NRT2.1 inter-individual variability in plants.PLoS genetics · 2025Article
- BdNRT2A and BdNRT3.2 Are the Major Components of the High-Affinity Nitrate Transport System inPlant direct · 2025Article
- Non-thermal plasma activated water is an effective nitrogen fertilizer alternative for Arabidopsis thaliana.PloS one · 2025Article
- Phosphoregulation in the N-terminus of NRT2.1 affects nitrate uptake by controlling the interaction of NRT2.1 with NAR2.1 and kinase HPCAL1 in Arabidopsis.Journal of experimental botany · 2024Article
- Facilitating Growth of Maize (Journal of agricultural and food chemistry · 2024Article
- Identification of candidate genes and residues for improving nitrogen use efficiency in the N-sensitive medicinal plant Panax notoginseng.BMC plant biology · 2024Article
- Functional analyses of the NRT2 family of nitrate transporters inFrontiers in plant science · 2024Review
- Root nitrate uptake in sugarcane (Saccharum spp.) is modulated by transcriptional and presumably posttranscriptional regulation of the NRT2.1/NRT3.1 transport system.Molecular genetics and genomics : MGG · 2022Article
- Genome-Wide Identification ofGenes · 2022Article
- New insights into the role of chrysanthemum calcineurin B-like interacting protein kinase CmCIPK23 in nitrate signaling in Arabidopsis roots.Scientific reports · 2022Article
- Genome-wide identification and characterization of the NPF genes provide new insight into low nitrogen tolerance inFrontiers in plant science · 2022Article
- Liberating nitrate transport activity.Nature plants · 2021Article
- A type 2C protein phosphatase activates high-affinity nitrate uptake by dephosphorylating NRT2.1.Nature plants · 2021Article
- The Physiological Implications ofAntioxidants (Basel, Switzerland) · 2020Review
- Small differences in root distributions allow resource niche partitioning.Ecology and evolution · 2020Article
- Physiological and Proteomic Dissection of the Responses of Two Contrasting Wheat Genotypes to Nitrogen Deficiency.International journal of molecular sciences · 2020Article
- Overexpression of the High-Affinity Nitrate TransporterInternational journal of molecular sciences · 2020Article
- Expression of the cassava nitrate transporterJournal of genetics · 2019Article
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6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Arabidopsis (Arabidopsis thaliana), the NRT2.1 gene codes for the main component of the root nitrate (NO(3)(-)) high-affinity transport system (HATS). Due to the strong correlation generally found between high-affinity root NO(3)(-) influx and NRT2.1 mRNA level, it has been postulated that transcriptional regulation of NRT2.1 is a key mechanism for modulation of the HATS activity. However, this hypothesis has never been demonstrated, and is challenged by studies suggesting the occurrence of posttranscriptional regulation at the NRT2.1 protein level. To unambiguously clarify the respective roles of transcriptional and posttranscriptional regulations of NRT2.1, we generated transgenic lines expressing a functional 35S::NRT2.1 transgene in an atnrt2.1 mutant background. Despite a high and constitutive NRT2.1 transcript accumulation in the roots, the HATS activity was still down-regulated in the 35S::NRT2.1 transformants in response to repressive nitrogen or dark treatments that strongly reduce NRT2.1 transcription and NO(3)(-) HATS activity in the wild type. In some treatments, this was associated with a decline of NRT2.1 protein abundance, indicating posttranscriptional regulation of NRT2.1. However, in other instances, NRT2.1 protein level remained constant. Changes in abundance of NAR2.1, a partner protein of NRT2.1, closely followed those of NRT2.1, and thus could not explain the close-to-normal regulation of the HATS in the 35S::NRT2.1 transformants. Even if in certain conditions the transcriptional regulation of NRT2.1 contributes to a limited extent to the control of the HATS, we conclude from this study that posttranscriptional regulation of NRT2.1 and/or NAR2.1 plays a predominant role in the control of the NO(3)(-) HATS in Arabidopsis.
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