ArticleThe ISME journal2012
High-throughput isotopic analysis of RNA microarrays to quantify microbial resource use.
Article in The ISME journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 65 citations in OpenAlex.
- Advances and perspectives of using stable isotope probing (SIP)-based technologies in contaminant biodegradation.Water research X · 2023Article
- NanoSIP: NanoSIMS Applications for Microbial Biology.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Community RNA-Seq: multi-kingdom responses to living versus decaying roots in soil.ISME communications · 2021Article
- Bridging Microbial Functional Traits With Localized Process Rates at Soil Interfaces.Frontiers in microbiology · 2021Review
- Distinct capabilities of different Gammaproteobacterial strains on utilizing small peptides in seawater.Scientific reports · 2020Article
- Stable Isotope Probing Identifies Bacterioplankton Lineages Capable of Utilizing Dissolved Organic Matter Across a Range of Bioavailability.Frontiers in microbiology · 2020Article
- Influence of Light on Particulate Organic Matter Utilization by Attached and Free-Living Marine Bacteria.Frontiers in microbiology · 2019Article
- Stable-Isotope Probing of Human and Animal Microbiome Function.Trends in microbiology · 2018Review
- Phylogenetically conserved resource partitioning in the coastal microbial loop.The ISME journal · 2017Article
- Quantitative imaging of deuterated metabolic tracers in biological tissues with nanoscale secondary ion mass spectrometry.International journal of mass spectrometry · 2017Article
- Light Regimes Shape Utilization of Extracellular Organic C and N in a Cyanobacterial Biofilm.mBio · 2016Article
- Microbial Surface Colonization and Biofilm Development in Marine Environments.Microbiology and molecular biology reviews : MMBR · 2016Review
- Quantitative microbial ecology through stable isotope probing.Applied and environmental microbiology · 2015Article
- Tracking heavy water (D2O) incorporation for identifying and sorting active microbial cells.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Quasi-simultaneous acquisition of nine secondary ions with seven detectors on NanoSIMS50L: application to biological samples.Surface and interface analysis : SIA · 2014Article
- Impact of single-cell genomics and metagenomics on the emerging view of extremophile "microbial dark matter".Extremophiles : life under extreme conditions · 2014Review
- Phylogenetic patterns in the microbial response to resource availability: amino acid incorporation in San Francisco Bay.PloS one · 2014Article
- Trait-based approaches for understanding microbial biodiversity and ecosystem functioning.Frontiers in microbiology · 2014Review
- Insights from quantitative metaproteomics and protein-stable isotope probing into microbial ecology.The ISME journal · 2013Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most microorganisms remain uncultivated, and typically their ecological roles must be inferred from diversity and genomic studies. To directly measure functional roles of uncultivated microbes, we developed Chip-stable isotope probing (SIP), a high-sensitivity, high-throughput SIP method performed on a phylogenetic microarray (chip). This approach consists of microbial community incubations with isotopically labeled substrates, hybridization of the extracted community rRNA to a microarray and measurement of isotope incorporation--and therefore substrate use--by secondary ion mass spectrometer imaging (NanoSIMS). Laboratory experiments demonstrated that Chip-SIP can detect isotopic enrichment of 0.5 atom % (13)C and 0.1 atom % (15)N, thus permitting experiments with short incubation times and low substrate concentrations. We applied Chip-SIP analysis to a natural estuarine community and quantified amino acid, nucleic acid or fatty acid incorporation by 81 distinct microbial taxa, thus demonstrating that resource partitioning occurs with relatively simple organic substrates. The Chip-SIP approach expands the repertoire of stable isotope-enabled methods available to microbial ecologists and provides a means to test genomics-generated hypotheses about biogeochemical function in any natural environment.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.