ArticleThe EMBO journal2012
Rescue of tropomyosin deficiency in Drosophila and human cancer cells by synaptopodin reveals a role of tropomyosin α in RhoA stabilization.
Article in The EMBO journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- The podocytes' inflammatory responses in experimental GN are independent of canonical MYD88-dependent toll-like receptor signaling.Scientific reports · 2024Article
- Synaptopodin Is Dispensable for Normal Podocyte Homeostasis but Is Protective in the Context of Acute Podocyte Injury.Journal of the American Society of Nephrology : JASN · 2020Article
- A Division of Labor between YAP and TAZ in Non-Small Cell Lung Cancer.Cancer research · 2020Article
- Gene Panel of Persister Cells as a Prognostic Indicator for Tumor Repopulation After Radiation.Frontiers in oncology · 2020Article
- Podocytes and the quest for precision medicines for kidney diseases.Pflugers Archiv : European journal of physiology · 2017Review
- Synaptopodin Is a Coincidence Detector of Tyrosine versus Serine/Threonine Phosphorylation for the Modulation of Rho Protein Crosstalk in Podocytes.Journal of the American Society of Nephrology : JASN · 2017Article
- Mutations in tropomyosin 4 underlie a rare form of human macrothrombocytopenia.The Journal of clinical investigation · 2017Article
- Article
- Podocytes … What's Under Yours? (Podocytes and Foot Processes and How They Change in Nephropathy).Frontiers in endocrinology · 2015Review
- Article
- A high-throughput screen identifies miRNA inhibitors regulating lung cancer cell survival and response to paclitaxel.RNA biology · 2013Article
- Essential role for synaptopodin in dendritic spine plasticity of the developing hippocampus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Proteasomal degradation of Nck1 but not Nck2 regulates RhoA activation and actin dynamics.Nature communications · 2013Article
- Calcium regulates podocyte actin dynamics.Seminars in nephrology · 2012Review
Corrections and comments
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Authors and funding
10 authors at 6 institutions in 2 countries.
Funding
Abstract
Tropomyosins are widespread actin-binding proteins that influence numerous cellular functions including actin dynamics, cell migration, tumour suppression, and Drosophila oocyte development. Synaptopodin is another actin-binding protein with a more restricted expression pattern in highly dynamic cell compartments such as kidney podocyte foot processes, where it promotes RhoA signalling by blocking the Smurf1-mediated ubiquitination of RhoA. Here, we show that synaptopodin has a shorter half-life but shares functional properties with the highly stable tropomyosin. Transgenic expression of synaptopodin restores oskar mRNA localization in Drosophila oocytes mutant for TmII, thereby rescuing germline differentiation and fertility. Synaptopodin restores stress fibres in tropomyosin-deficient human MDA-MB 231 breast cancer cells and TPMα-depleted fibroblasts. Gene silencing of TPMα but not TPMβ causes loss of stress fibres by promoting Smurf1-mediated ubiquitination and proteasomal degradation of RhoA. Functionally, overexpression of synaptopodin or RhoA(K6,7R) significantly reduces MDA-MB 231 cell migration. Our findings elucidate RhoA stabilization by structurally unrelated actin-binding proteins as a conserved mechanism for regulation of stress fibre dynamics and cell motility in a cell type-specific fashion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.