ArticleApplied and environmental microbiology2012
Cloning and characterization of the polyether salinomycin biosynthesis gene cluster of Streptomyces albus XM211.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- A system of paired polyether epoxide hydrolases enables a mouldable enzyme for consecutive ring cyclization cascades.Nature chemistry · 2026Article
- P450 Enzyme LyoI Performs Hydro-2,2'-Bifuran Oxidation in the Polyether Ionophore Lysocellin.Angewandte Chemie (International ed. in English) · 2025Article
- Review
- An efficient method for targeted cloning of large DNA fragments from Streptomyces.Applied microbiology and biotechnology · 2023Article
- Salinomycin biosynthesis reversely regulates the β-oxidation pathway in Streptomyces albus by carrying a 3-hydroxyacyl-CoA dehydrogenase gene in its biosynthetic gene cluster.Microbial biotechnology · 2022Article
- Secondary Metabolites and Biosynthetic Gene Clusters Analysis of Deep-Sea Hydrothermal Vent-DerivedMarine drugs · 2022Article
- Comparative Transcriptome-Based Mining of Genes Involved in the Export of Polyether Antibiotics for Titer Improvement.Antibiotics (Basel, Switzerland) · 2022Article
- Reconstitution of a mini-gene cluster combined with ribosome engineering led to effective enhancement of salinomycin production in Streptomyces albus.Microbial biotechnology · 2021Article
- Biodiversity of Secondary Metabolites Compounds Isolated from Phylum Actinobacteria and Its Therapeutic Applications.Molecules (Basel, Switzerland) · 2021Review
- Biosynthesis of Chlorinated Lactylates inJournal of natural products · 2021Article
- A Hierarchical Network of Four Regulatory Genes Controlling Production of the Polyene Antibiotic Candicidin inApplied and environmental microbiology · 2020Article
- Genomics-driven discovery of the biosynthetic gene cluster of maduramicin and its overproduction in Actinomadura sp. J1-007.Journal of industrial microbiology & biotechnology · 2020Article
- Actinomycete-Derived Polyketides as a Source of Antibiotics and Lead Structures for the Development of New Antimicrobial Drugs.Antibiotics (Basel, Switzerland) · 2019Review
- 3-Ketoacyl-ACP synthase (KAS) III homologues and their roles in natural product biosynthesis.MedChemComm · 2019Review
- The biosynthetic pathway to ossamycin, a macrocyclic polyketide bearing a spiroacetal moiety.PloS one · 2019Article
- Combinatorial Effect of ARTP Mutagenesis and Ribosome Engineering on an Industrial Strain ofFrontiers in bioengineering and biotechnology · 2019Article
- Activation of microbial secondary metabolic pathways: Avenues and challenges.Synthetic and systems biotechnology · 2018Review
- An inhibitory compound produced by a soil isolate of Rhodococcus has strong activity against the veterinary pathogen R. equi.PloS one · 2018Article
- Elucidation of the Cryptic Methyl Group Epimerase Activity of Dehydratase Domains from Modular Polyketide Synthases Using a Tandem Modules Epimerase Assay.Journal of the American Chemical Society · 2017Article
- The Uncommon Enzymology of Cis-Acyltransferase Assembly Lines.Chemical reviews · 2017Review
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Salinomycin is widely used in animal husbandry as a food additive due to its antibacterial and anticoccidial activities. However, its biosynthesis had only been studied by feeding experiments with isotope-labeled precursors. A strategy with degenerate primers based on the polyether-specific epoxidase sequences was successfully developed to clone the salinomycin gene cluster. Using this strategy, a putative epoxidase gene, slnC, was cloned from the salinomycin producer Streptomyces albus XM211. The targeted replacement of slnC and subsequent trans-complementation proved its involvement in salinomycin biosynthesis. A 127-kb DNA region containing slnC was sequenced, including genes for polyketide assembly and release, oxidative cyclization, modification, export, and regulation. In order to gain insight into the salinomycin biosynthesis mechanism, 13 gene replacements and deletions were conducted. Including slnC, 7 genes were identified as essential for salinomycin biosynthesis and putatively responsible for polyketide chain release, oxidative cyclization, modification, and regulation. Moreover, 6 genes were found to be relevant to salinomycin biosynthesis and possibly involved in precursor supply, removal of aberrant extender units, and regulation. Sequence analysis and a series of gene replacements suggest a proposed pathway for the biosynthesis of salinomycin. The information presented here expands the understanding of polyether biosynthesis mechanisms and paves the way for targeted engineering of salinomycin activity and productivity.
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