ArticleNucleic acids research2012
Functional binding of hexanucleotides to 3C protease of hepatitis A virus.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Single-stranded nucleic acid binding enhances the in vitro catalytic activity of Chikungunya virus nsP2 protease.BBA advances · 2026Article
- Hepatitis A: Viral Structure, Classification, Life Cycle, Clinical Symptoms, Diagnosis Error, and Vaccination.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2023Review
- Unlocking G-Quadruplexes as Targets and Tools against COVID-19.Chinese journal of chemistry · 2022Article
- Therapeutic Advances in Viral Hepatitis A-E.Advances in therapy · 2022Review
- Hepatitis A: Epidemiology, High-Risk Groups, Prevention and Research on Antiviral Treatment.Viruses · 2021Review
- G4 Matters-The Influence of G-Quadruplex Structural Elements on the Antiproliferative Properties of G-Rich Oligonucleotides.International journal of molecular sciences · 2021Article
- Hepatitis A: Epidemiology, Natural History, Unusual Clinical Manifestations, and Prevention.Gastroenterology clinics of North America · 2020Review
- G-Quadruplex-Forming Aptamers-Characteristics, Applications, and Perspectives.Molecules (Basel, Switzerland) · 2019Review
- Article
- G-quadruplex-interacting compounds alter latent DNA replication and episomal persistence of KSHV.Nucleic acids research · 2016Article
- Direct-acting Antivirals and Host-targeting Agents against the Hepatitis A Virus.Journal of clinical and translational hepatology · 2015Review
- G-quadruplexes in viruses: function and potential therapeutic applications.Nucleic acids research · 2014Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oligonucleotides as short as 6 nt in length have been shown to bind specifically and tightly to proteins and affect their biological function. Yet, sparse structural data are available for corresponding complexes. Employing a recently developed hexanucleotide array, we identified hexadeoxyribonucleotides that bind specifically to the 3C protease of hepatitis A virus (HAV 3C(pro)). Inhibition assays in vitro identified the hexanucleotide 5'-GGGGGT-3' (G(5)T) as a 3C(pro) protease inhibitor. Using (1)H NMR spectroscopy, G(5)T was found to form a G-quadruplex, which might be considered as a minimal aptamer. With the help of (1)H, (15)N-HSQC experiments the binding site for G(5)T was located to the C-terminal β-barrel of HAV 3C(pro). Importantly, the highly conserved KFRDI motif, which has previously been identified as putative viral RNA binding site, is not part of the G(5)T-binding site, nor does G(5)T interfere with the binding of viral RNA. Our findings demonstrate that sequence-specific nucleic acid-protein interactions occur with oligonucleotides as small as hexanucleotides and suggest that these compounds may be of pharmaceutical relevance.
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Registered trials
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