ArticleNucleic acids research2012
Structural and functional analysis of the archaeal endonuclease Nob1.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 74 citations in OpenAlex.
- Article
- Archaeal RNA processing and regulation: expanding the functional landscape.Microbiology and molecular biology reviews : MMBR · 2025Review
- RNase W, a conserved ribonuclease family with a novel active site.Nucleic acids research · 2024Article
- An improved CRISPR and CRISPR interference (CRISPRi) toolkit for engineering the model methanogenic archaeon Methanococcus maripaludis.Microbial cell factories · 2024Article
- The kinase Rio1 and a ribosome collision-dependent decay pathway survey the integrity of 18S rRNA cleavage.PLoS biology · 2024Article
- In silico approaches for the identification of potential allergens among hypothetical proteins from Alternaria alternata and its functional annotation.Scientific reports · 2024Article
- Domain definition and preliminary functional exploration of the endonuclease NOBP-1 in Strongyloides stercoralis.Parasites & vectors · 2023Article
- Nanopore-based RNA sequencing deciphers the formation, processing, and modification steps of rRNA intermediates in archaea.RNA (New York, N.Y.) · 2023Article
- Quality control ensures fidelity in ribosome assembly and cellular health.The Journal of cell biology · 2023Review
- Endonucleolytic processing plays a critical role in the maturation of ribosomal RNA inRNA biology · 2023Article
- Looking through the Lens of the Ribosome Biogenesis Evolutionary History: Possible Implications for Archaeal Phylogeny and Eukaryogenesis.Molecular biology and evolution · 2022Article
- Capillary Electrophoresis-Based Functional Genomics Screening to Discover Novel Archaeal DNA Modifying Enzymes.Applied and environmental microbiology · 2022Article
- A Comparative Perspective on Ribosome Biogenesis: Unity and Diversity Across the Tree of Life.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Insights into synthesis and function of KsgA/Dim1-dependent rRNA modifications in archaea.Nucleic acids research · 2021Article
- Ribosome Biogenesis in Archaea.Frontiers in microbiology · 2021Review
- MicroRNA-363-3p downregulation in papillary thyroid cancer inhibits tumor progression by targeting NOB1.Journal of investigative medicine : the official publication of the American Federation for Clinical Research · 2021Article
- Comprehensive analysis of the pre-ribosomal RNA maturation pathway in a methanoarchaeon exposes the conserved circularization and linearization mode in archaea.RNA biology · 2020Article
- MiR-363 suppresses cell migration, invasion, and epithelial-mesenchymal transition of osteosarcoma by binding to NOB1.World journal of surgical oncology · 2020Observational
- A versatile cis-acting element reporter system to study the function, maturation and stability of ribosomal RNA mutants in archaea.Nucleic acids research · 2020Article
- Identification of the 3-amino-3-carboxypropyl (acp) transferase enzyme responsible for acp3U formation at position 47 in Escherichia coli tRNAs.Nucleic acids research · 2020Article
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Authors and funding
11 authors at 1 institution in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eukaryotic ribosome biogenesis requires the concerted action of numerous ribosome assembly factors, for most of which structural and functional information is currently lacking. Nob1, which can be identified in eukaryotes and archaea, is required for the final maturation of the small subunit ribosomal RNA in yeast by catalyzing cleavage at site D after export of the preribosomal subunit into the cytoplasm. Here, we show that this also holds true for Nob1 from the archaeon Pyrococcus horikoshii, which efficiently cleaves RNA-substrates containing the D-site of the preribosomal RNA in a manganese-dependent manner. The structure of PhNob1 solved by nuclear magnetic resonance spectroscopy revealed a PIN domain common with many nucleases and a zinc ribbon domain, which are structurally connected by a flexible linker. We show that amino acid residues required for substrate binding reside in the PIN domain whereas the zinc ribbon domain alone is sufficient to bind helix 40 of the small subunit rRNA. This suggests that the zinc ribbon domain acts as an anchor point for the protein on the nascent subunit positioning it in the proximity of the cleavage site.
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