ArticleMolecular pharmacology2012
Protein kinase C regulation of 12-lipoxygenase-mediated human platelet activation.
Article in Molecular pharmacology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- Article
- Role of annexin A7 in the occurrence and progression of coronary atherosclerosis: a narrative review.Cardiovascular diagnosis and therapy · 2025Review
- Bioactive lipid regulation of platelet function, hemostasis, and thrombosis.Pharmacology & therapeutics · 2023Review
- TRP Channels as Sensors of Aldehyde and Oxidative Stress.Biomolecules · 2021Review
- Antimicrobial Effects of Thymosin Beta-4 and Ciprofloxacin Adjunctive Therapy inInternational journal of molecular sciences · 2020Article
- Evaluation of the in vitro effects of commercial herbal preparations significant in African traditional medicine on platelets.BMC complementary and alternative medicine · 2019Article
- Platelet Signaling and Disease: Targeted Therapy for Thrombosis and Other Related Diseases.Pharmacological reviews · 2018Review
- First Selective 12-LOX Inhibitor, ML355, Impairs Thrombus Formation and Vessel Occlusion In Vivo With Minimal Effects on Hemostasis.Arteriosclerosis, thrombosis, and vascular biology · 2017Article
- Who is the real 12-HETrE?Prostaglandins & other lipid mediators · 2017Review
- The expansive role of oxylipins on platelet biology.Journal of molecular medicine (Berlin, Germany) · 2017Review
- Mammalian lipoxygenases and their biological relevance.Biochimica et biophysica acta · 2015Review
- Common mechanisms of Alzheimer's disease and ischemic stroke: the role of protein kinase C in the progression of age-related neurodegeneration.Journal of Alzheimer's disease : JAD · 2015Review
- Article
- Synthesis and structure-activity relationship studies of 4-((2-hydroxy-3-methoxybenzyl)amino)benzenesulfonamide derivatives as potent and selective inhibitors of 12-lipoxygenase.Journal of medicinal chemistry · 2014Article
- The emerging role of oxylipins in thrombosis and diabetes.Frontiers in pharmacology · 2014Review
- 12-hydroxyeicosatetraenoic acid is associated with variability in aspirin-induced platelet inhibition.Journal of inflammation (London, England) · 2014Article
- Racial differences in human platelet PAR4 reactivity reflect expression of PCTP and miR-376c.Nature medicine · 2013Article
- 12-lipoxygenase activity plays an important role in PAR4 and GPVI-mediated platelet reactivity.Thrombosis and haemostasis · 2013Article
- Pseudoperoxidase investigations of hydroperoxides and inhibitors with human lipoxygenases.Bioorganic & medicinal chemistry · 2013Article
- Lipoxygenase: an emerging target for stroke therapy.CNS & neurological disorders drug targets · 2013Review
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
Platelet activation is important in the regulation of hemostasis and thrombosis. Uncontrolled activation of platelets may lead to arterial thrombosis, which is a major cause of myocardial infarction and stroke. After activation, metabolism of arachidonic acid (AA) by 12-lipoxygenase (12-LOX) may play a significant role in regulating the degree and stability of platelet activation because inhibition of 12-LOX significantly attenuates platelet aggregation in response to various agonists. Protein kinase C (PKC) activation is also known to be an important regulator of platelet activity. Using a newly developed selective inhibitor for 12-LOX and a pan-PKC inhibitor, we investigated the role of PKC in 12-LOX-mediated regulation of agonist signaling in the platelet. To determine the role of PKC within the 12-LOX pathway, a number of biochemical endpoints were measured, including platelet aggregation, calcium mobilization, and integrin activation. Inhibition of 12-LOX or PKC resulted in inhibition of dense granule secretion and attenuation of both aggregation and αIIbβ(3) activation. However, activation of PKC downstream of 12-LOX inhibition rescued agonist-induced aggregation and integrin activation. Furthermore, inhibition of 12-LOX had no effect on PKC-mediated aggregation, indicating that 12-LOX is upstream of PKC. These studies support an essential role for PKC downstream of 12-LOX activation in human platelets and suggest 12-LOX as a possible target for antiplatelet therapy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.