ArticleClinical and vaccine immunology : CVI2012
Identification of immunogenic and virulence-associated Campylobacter jejuni proteins.
Article in Clinical and vaccine immunology : CVI, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 22 citations in OpenAlex.
- Application of TraDIS to define the core essential genome of Campylobacter jejuni and Campylobacter coli.BMC microbiology · 2023Article
- Bacteroides fragilis fucosidases facilitate growth and invasion of Campylobacter jejuni in the presence of mucins.Cellular microbiology · 2020Article
- Domestication of Campylobacter jejuni NCTC 11168.Microbial genomics · 2019Article
- A proteome-wide screen of Campylobacter jejuni using protein microarrays identifies novel and conformational antigens.PloS one · 2019Article
- Engineering the Campylobacter jejuni N-glycan to create an effective chicken vaccine.Scientific reports · 2016Article
- Chicken Anti-Campylobacter Vaccine - Comparison of Various Carriers and Routes of Immunization.Frontiers in microbiology · 2016Article
- Efficient inference of recombination hot regions in bacterial genomes.Molecular biology and evolution · 2014Article
- The fibronectin-binding motif within FlpA facilitates Campylobacter jejuni adherence to host cell and activation of host cell signaling.Emerging microbes & infections · 2013Article
- Article
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
With the aim of identifying proteins important for host interaction and virulence, we have screened an expression library of NCTC 11168 Campylobacter jejuni genes for highly immunogenic proteins. A commercial C. jejuni open reading frame (ORF) library consisting of more than 1,600 genes was transformed into the Escherichia coli expression strain BL21(DE3), resulting in 2,304 clones. This library was subsequently screened for immunogenic proteins using antibodies raised in rabbit against a clinical isolate of C. jejuni; this resulted in 52 highly reactive clones representing 25 different genes after sequencing. Selected candidate genes were inactivated in C. jejuni NCTC 11168, and the virulence was examined using INT 407 epithelial cell line and motility, biofilm, autoagglutination, and serum resistance assays. These investigations revealed C. jejuni antigen 0034c (Cj0034c) to be a novel virulence factor and support the usefulness of the method. Further, several antigens were tested as vaccine candidates in two mouse models, in which Cj0034c, Cj0404, and Cj0525c resulted in a reduction of invasion in spleen and liver after challenge.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.