ArticleToxicology and applied pharmacology2012
Di (2-ethylhexyl) phthalate inhibits growth of mouse ovarian antral follicles through an oxidative stress pathway.
Article in Toxicology and applied pharmacology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers, 1 of them a synthesis that pooled it.
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Who cites it
68 citing papers in PubMed, 1 synthesis or guideline pooled it, 151 citations in OpenAlex.
- Reproductive and developmental effects of phthalate diesters in females.Critical reviews in toxicology · 2013Pooled it
- Human umbilical cord stem cell conditioned medium versus serum-free culture medium in the treatment of cryopreserved human ovarian tissues in in-vitro culture: a randomized controlled trial.Stem cell research & therapy · 2017Trial
- Lipid dysregulation in mouse ovarian antral follicles after oral exposure to a human relevant mixture of three phthalates.Toxicology · 2026Article
- Effects of Phthalate Metabolite Mixture Exposure on Mouse Oocyte Development.bioRxiv : the preprint server for biology · 2026Article
- An environmentally relevant phthalate mixture impairs ovulatory prostaglandin and progesterone receptor pathways in human granulosa cells in vitro.Environment international · 2026Article
- Living in a chemical cocktail: real-world mixture exposures and their impact on ovarian function.The Journal of reproduction and development · 2026Review
- Mitigating the environmental effects of healthcare: the role of the endocrinologist.Nature reviews. Endocrinology · 2025Review
- Prenatal exposure to an environmentally relevant phthalate mixture alters oxidative stress, apoptosis, cell cycle regulators, and steroidogenic factors in the ovaries of F1 mice.Reproductive toxicology (Elmsford, N.Y.) · 2025Article
- Phthalates are detected in the follicular fluid of adolescents and oocyte donors with associated changes in the cumulus cell transcriptome.F&S science · 2025Article
- Dietary exposure to di(2-ethylhexyl) phthalate for 6 months alters markers of female reproductive aging in mice†.Biology of reproduction · 2025Article
- Novel Molecular Mechanisms Underlying the Ameliorative Effect of Platelet-Rich Plasma against Electron Radiation-Induced Premature Ovarian Failure.International journal of molecular sciences · 2024Article
- Morin hydrate ameliorates Di-2-ethylhexyl phthalate (DEHP) induced hepatotoxicity in a mouse model via TNF-α and NF-κβ signaling.3 Biotech · 2024Article
- Serum Phthalate Concentrations and Biomarkers of Oxidative Stress in Adipose Tissue in a Spanish Adult Cohort.Environmental science & technology · 2024Article
- The possible role of the endocrine disrupting chemicals on the premature and early menopause associated with the altered oxidative stress metabolism.Frontiers in endocrinology · 2023Article
- Urinary phthalate metabolite concentrations and hot flash outcomes: Longitudinal associations in the Midlife Women's Health Study.Environmental research · 2023Article
- The effects of endocrine-disrupting chemicals on ovarian- and ovulation-related fertility outcomes.Molecular reproduction and development · 2022Review
- Mapping DEHP to the adverse outcome pathway network for human female reproductive toxicity.Archives of toxicology · 2022Article
- Diethyl phthalate (DEP) perturbs nitrogen metabolism in Saccharomyces cerevisiae.Scientific reports · 2022Article
- Review
- Quercetin ameliorates Di (2-ethylhexyl) phthalate-induced nephrotoxicity by inhibiting NF-κB signaling pathway.Toxicology research · 2022Article
8 more citing papers are in PubMed but not listed here.
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5 authors at 2 institutions in 1 country.
Funding
Abstract
Di (2-ethylhexyl) phthalate (DEHP) is a plasticizer that has been shown to inhibit growth of mouse antral follicles, however, little is known about the mechanisms by which DEHP does so. Oxidative stress has been linked to follicle growth inhibition as well as phthalate-induced toxicity in non-ovarian tissues. Thus, we hypothesized that DEHP causes oxidative stress and that this leads to inhibition of the growth of antral follicles. To test this hypothesis, antral follicles isolated from CD-1 mice (age 31-35days) were cultured with vehicle control (dimethylsulfoxide [DMSO]) or DEHP (1-100μg/ml)±N-acetyl cysteine (NAC, an antioxidant at 0.25-1mM). During culture, follicles were measured daily. At the end of culture, follicles were collected and processed for in vitro reactive oxygen species (ROS) assays to measure the presence of free radicals or for measurement of the expression and activity of various key antioxidant enzymes: Cu/Zn superoxide dismutase (SOD1), glutathione peroxidase (GPX) and catalase (CAT). The results indicate that DEHP inhibits the growth of follicles compared to DMSO control and that NAC (0.25-1mM) blocks the ability of DEHP to inhibit follicle growth. Furthermore, DEHP (10μg/ml) significantly increases ROS levels and reduces the expression and activity of SOD1 compared to DMSO controls, whereas NAC (0.5mM) rescues the effects of DEHP on ROS levels and SOD1. However, the expression and activity of GPX and CAT were not affected by DEHP treatment. Collectively, these data suggest that DEHP inhibits follicle growth by inducing production of ROS and by decreasing the expression and activity of SOD1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.