ArticleMicrobial cell factories2011
High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transfer.
Article in Microbial cell factories, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 63 citations in OpenAlex.
- Development of a Jacketed Breathable Shake Flask With Process Monitoring, Control, and Bioreactor-Like Performance.Biotechnology and bioengineering · 2026Article
- Foam Formation in Shake Flasks and Its Consequences.Engineering in life sciences · 2025Article
- Optimizing recombinant mini proinsulin production via response surface method and microbioreactor screening.PloS one · 2025Article
- Multimeric structure of a subfamily III haloalkane dehalogenase-like enzyme solved by combination of cryo-EM and x-ray crystallography.Protein science : a publication of the Protein Society · 2023Article
- High cell density cultivation ofBiotechnology reports (Amsterdam, Netherlands) · 2022Article
- Media optimization for SHuffle T7 Escherichia coli expressing SUMO-Lispro proinsulin by response surface methodology.BMC biotechnology · 2022Article
- Implementation of Perforated Concentric Ring Walls Considerably Improves Gas-Liquid Mass Transfer of Shaken Bioreactors.Frontiers in bioengineering and biotechnology · 2022Article
- Production of soluble regulatory hydrogenase from Ralstonia eutropha in Escherichia coli using a fed-batch-based autoinduction system.Microbial cell factories · 2021Article
- Mutations in sigma 70 transcription factor improves expression of functional eukaryotic membrane proteins in Escherichia coli.Scientific reports · 2019Article
- Accelerated pharmaceutical protein development with integrated cell free expression, purification, and bioconjugation.Scientific reports · 2018Article
- Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask.Microbial cell factories · 2018Article
- The fed-batch principle for the molecular biology lab: controlled nutrient diets in ready-made media improve production of recombinant proteins in Escherichia coli.Microbial cell factories · 2016Review
- Homogeneity and heterogeneity in amylase production by Bacillus subtilis under different growth conditions.Microbial cell factories · 2016Article
- Assessment of configurations and chemistries of bridged nucleic acids-containing oligomers as external guide sequences: a methodology for inhibition of expression of antibiotic resistance genes.Biology methods & protocols · 2016Article
- Easy to use and reliable technique for online dissolved oxygen tension measurement in shake flasks using infrared fluorescent oxygen-sensitive nanoparticles.Microbial cell factories · 2016Article
- Production of the polyketide 6-deoxyerythronolide B in the heterologous host Bacillus subtilis.Applied microbiology and biotechnology · 2016Article
- Expression of the functional recombinant human glycosyltransferase GalNAcT2 in Escherichia coli.Microbial cell factories · 2015Article
- Protein production in Yarrowia lipolytica via fusion to the secreted lipase Lip2p.Molecular biotechnology · 2014Article
- High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli.Microbial cell factories · 2013Article
- Effect of culture medium, host strain and oxygen transfer on recombinant Fab antibody fragment yield and leakage to medium in shaken E. coli cultures.Microbial cell factories · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This report describes the combined use of an enzyme-based glucose release system (EnBase®) and high-aeration shake flask (Ultra Yield Flask™). The benefit of this combination is demonstrated by over 100-fold improvement in the active yield of recombinant alcohol dehydrogenase expressed in E. coli. Compared to Terrific Broth and ZYM-5052 autoinduction medium, the EnBase system improved yield mainly through increased productivity per cell. Four-fold increase in oxygen transfer by the Ultra Yield Flask contributed to higher cell density with EnBase but not with the other tested media, and consequently the product yield per ml of EnBase culture was further improved.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.