ArticleBMC infectious diseases2011
Molecular detection and speciation of pathogenic Leptospira spp. in blood from patients with culture-negative leptospirosis.
Article in BMC infectious diseases, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Diagnostic accuracy of genetic markers and nucleic acid techniques for the detection of Leptospira in clinical samples: A meta-analysis.PLoS neglected tropical diseases · 2020Pooled it
- Molecular epidemiology of pathogenic Leptospira spp. in Thailand during 2024-2025, with detection of Leptospira weilii in human clinical specimens.PLoS neglected tropical diseases · 2026Article
- Retrospective Molecular Detection and Characterization of PathogenicTropical medicine and infectious disease · 2026Article
- Pathogenic Leptospira species identified in dogs and cats during neutering in Thailand.PLoS neglected tropical diseases · 2026Article
- Leptospirosis in Southeast Asia: Investigating Seroprevalence, Transmission Patterns, and Diagnostic Challenges.Tropical medicine and infectious disease · 2026Review
- Leptospirosis Risk Assessment in Rodent Populations and Environmental Reservoirs in Humanitarian Aid Settings in Thailand.Microorganisms · 2024Article
- Improved Antibody Detection for Canine Leptospirosis: ELISAs Modified Using Local Leptospiral Serovar Isolates from Asymptomatic Dogs.Animals : an open access journal from MDPI · 2024Article
- A rare case of anti-DPPX encephalitis combined with neuroleptospirosis.BMC neurology · 2024Article
- Utility evaluation of two molecular methods forHeliyon · 2023Article
- Application of Multilocus Sequence Typing for the Characterization ofTropical medicine and infectious disease · 2023Article
- Predictors of severe leptospirosis: a multicentre observational study from Central Malaysia.BMC infectious diseases · 2021Observational
- Potentially PathogenicTropical medicine and infectious disease · 2020Article
- Evaluation of three qPCR for the detection of pathogenic leptospires in domestic animals in NicaraguaBiomedica : revista del Instituto Nacional de Salud · 2020Article
- Low Prevalence ofTropical medicine and infectious disease · 2020Article
- Seroprevalence ofJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020Article
- Leptospira interrogans and Leptospira kirschneri are the dominant Leptospira species causing human leptospirosis in Central Malaysia.PLoS neglected tropical diseases · 2020Article
- Leptospirosis in an asplenic patient -case report.BMC infectious diseases · 2020Article
- Leptospiral Culture without 5'-Fluorouracil Revealed ImprovedInternational journal of environmental research and public health · 2020Article
- Combined PCR and MAT improves the early diagnosis of the biphasic illness leptospirosis.PloS one · 2020Observational
- Whole cell matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identification of Leptospira spp. in Thailand and Lao PDR.PLoS neglected tropical diseases · 2019Article
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8 authors.
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Abstract
backgroundPathogenic Leptospira spp. present in the blood of patients with leptospirosis during the first week of symptoms can be detected using culture or PCR. A proportion of patients who are positive by PCR are negative by culture. Leptospira spp. are fastidious bacteria, and we hypothesized that a false-negative culture result may represent infection with a distinct bacterial subset that fail to grow in standard culture medium.
methodsWe evaluated our hypothesis during a prospective study of 418 consecutive patients presenting to a hospital in northeast Thailand with an acute febrile illness. Admission blood samples were taken for Leptospira culture and PCR. A single tube nested PCR that amplified a region of the rrs gene was developed and applied, amplicons sequenced and a phylogenetic tree reconstructed.
results39/418 (9%) patients were culture-positive for Leptospira spp., and 81/418 (19%) patients were culture-negative but rrs PCR-positive. The species associated with culture-positive leptospirosis (37 L. interrogans and 2 L. borgpetersenii) were comparable to those associated with culture-negative, PCR-positive leptospirosis (76 L. interrogans, 4 L. borgpetersenii, 1 unidentified, possibly new species).
conclusionMolecular speciation failed to identify a unique bacterial subset in patients with culture-negative, PCR-positive leptospirosis. The rate of false-negative culture was high, and we speculate that antibiotic pre-treatment is the most likely explanation for this.
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