ArticleAnalytical chemistry2012
Enhanced Gaussia luciferase blood assay for monitoring of in vivo biological processes.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 18 citations in OpenAlex.
- A plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassays.Nature communications · 2021Article
- Novel targeted mtLivin nanoparticles treatment for disseminated diffuse large B-cell lymphoma.Oncogene · 2021Article
- A cerebellopontine angle mouse model for the investigation of tumor biology, hearing, and neurological function in NF2-related vestibular schwannoma.Nature protocols · 2019Article
- A cerebellar window for intravital imaging of normal and disease states in mice.Nature protocols · 2017Article
- Exogenous marker-engineered mesenchymal stem cells detect cancer and metastases in a simple blood assay.Stem cell research & therapy · 2015Article
- In vivo cell tracking with bioluminescence imaging.Nuclear medicine and molecular imaging · 2015Review
- MicroRNA-26a promotes anoikis in human hepatocellular carcinoma cells by targeting alpha5 integrin.Oncotarget · 2015Article
- Bioluminescence: a versatile technique for imaging cellular and molecular features.MedChemComm · 2014Article
- Directed molecular evolution reveals Gaussia luciferase variants with enhanced light output stability.Analytical chemistry · 2013Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Secreted Gaussia luciferase (Gluc) has been shown to be a useful tool for ex vivo monitoring of in vivo biological processes. The Gluc level in the blood was used to detect tumor growth, metastasis and response to therapy, gene transfer, and circulating cells viability, as well as transcription factors activation, complementing in vivo bioluminescence imaging. The sensitivity of the Gluc blood assay is limited due to the absorption of blue light by pigmented molecules such as hemoglobin, resulting in quenching of the signal and therefore lower sensitivity. To overcome this problem, we designed an alternative microtiter well-based binding assay in which Gluc is captured first from blood using a specific antibody followed by the addition of coelenterazine and signal acquisition using a luminometer. This assay showed to be over 1 order of magnitude more sensitive in detecting Gluc in the blood as compared to the direct Gluc blood assay enhancing ex vivo monitoring of biological processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.