ArticleMarine biotechnology (New York, N.Y.)2012
In vivo RNA interference of a gonad-specific transforming growth factor-β in the Pacific oyster Crassostrea gigas.
Article in Marine biotechnology (New York, N.Y.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 40 citations in OpenAlex.
- Implications of Endogenous Small Regulatory RNAs Survey in Mollusks on Gene Silencing Approaches.Marine biotechnology (New York, N.Y.) · 2025Article
- Article
- RNAi based transcriptome suggests genes potentially regulated by HSF1 in the Pacific oyster Crassostrea gigas under thermal stress.BMC genomics · 2019Article
- Morphological and molecular criteria allow the identification of putative germ stem cells in a lophotrochozoan, the Pacific oyster Crassostrea gigas.Histochemistry and cell biology · 2019Article
- Trojan Horse Strategy for Non-invasive Interference of Clock Gene in the Oyster Crassostrea gigas.Marine biotechnology (New York, N.Y.) · 2017Article
- Identification and expression of cysteine sulfinate decarboxylase, possible regulation of taurine biosynthesis in Crassostrea gigas in response to low salinity.Scientific reports · 2017Article
- Insights into molecular features of Venerupis decussata oocytes: a microarray-based study.PloS one · 2014Article
- Sequencing and characterization of striped venus transcriptome expand resources for clam fishery genetics.PloS one · 2012Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the role of oyster gonadal TGFβ (og-TGFβ) in the reproduction of Crassostrea gigas, using an in vivo RNA interference approach. We designed double-stranded RNA targeting og-TGFβ, which is specifically expressed in the somatic cells surrounding germ cells in the gonad of both male and female oysters. In vivo injection of this og-TGFβ dsRNA into the gonad led to knock-down phenotypes for both sexes, with significant reduction (77.52% relative to controls) of the gonad area, lowered reproductive effort and germ cell under-proliferation. Interestingly, half of the injected females halted their vitellogenesis, since we were only able to observe pre-vitellogenic oocytes. In addition, apoptotic germ cells and haemocytes infiltrated into the gonad, likely as part of the active resorption of degenerating germ cells. Conversely, males showed a normal phenotype at the cellular level, with spermatids and spermatozoids observed in the gonads of control and injected males. As a result, og-TGFβ appears to play an essential role in C. gigas germ cell development by functioning as an activator of germ cell proliferation in both male and female oysters and vitellogenesis in females.
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Registered trials
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