ArticleMolecular systems biology2011
Overexpression limits of fission yeast cell-cycle regulators in vivo and in silico.
Article in Molecular systems biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 16 citations in OpenAlex.
- Network topology creates independent control of multiple checkpoints in the cell cycle system.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Nuclear size and physical properties of the nucleoplasm are determined by colloid osmotic pressure at the nuclear envelope.bioRxiv : the preprint server for biology · 2026Article
- Massive expression of cysteine-containing proteins causes abnormal elongation of yeast cells by perturbing the proteasome.G3 (Bethesda, Md.) · 2022Article
- Development of an experimental method of systematically estimating protein expression limits in HEK293 cells.Scientific reports · 2020Article
- Quantitative Systems Biology to decipher design principles of a dynamic cell cycle network: the "Maximum Allowable mammalian Trade-Off-Weight" (MAmTOW).NPJ systems biology and applications · 2017Review
- Cellular growth defects triggered by an overload of protein localization processes.Scientific reports · 2016Article
- Quantitative nature of overexpression experiments.Molecular biology of the cell · 2015Article
- Cdk1 promotes cytokinesis in fission yeast through activation of the septation initiation network.Molecular biology of the cell · 2014Article
- Evaluation of the lower protein limit in the budding yeast Saccharomyces cerevisiae using TIPI-gTOW.BMC systems biology · 2014Article
- Identification of dosage-sensitive genes in Saccharomyces cerevisiae using the genetic tug-of-war method.Genome research · 2013Article
- Article
- Relationships between cell cycle regulator gene copy numbers and protein expression levels in Schizosaccharomyces pombe.PloS one · 2013Article
- Dynamics of SIN asymmetry establishment.PLoS computational biology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cellular systems are generally robust against fluctuations of intracellular parameters such as gene expression level. However, little is known about expression limits of genes required to halt cellular systems. In this study, using the fission yeast Schizosaccharomyces pombe, we developed a genetic 'tug-of-war' (gTOW) method to assess the overexpression limit of certain genes. Using gTOW, we determined copy number limits for 31 cell-cycle regulators; the limits varied from 1 to >100. Comparison with orthologs of the budding yeast Saccharomyces cerevisiae suggested the presence of a conserved fragile core in the eukaryotic cell cycle. Robustness profiles of networks regulating cytokinesis in both yeasts (septation-initiation network (SIN) and mitotic exit network (MEN)) were quite different, probably reflecting differences in their physiologic functions. Fragility in the regulation of GTPase spg1 was due to dosage imbalance against GTPase-activating protein (GAP) byr4. Using the gTOW data, we modified a mathematical model and successfully reproduced the robustness of the S. pombe cell cycle with the model.
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