ArticleInfection and immunity2012
Effects of amino acid supplementation on porin expression and ToxR levels in Vibrio cholerae.
Article in Infection and immunity, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 43 citations in OpenAlex.
- Bile-mediated ToxS homodimerization provides a model for ToxRS periplasmic interactions.bioRxiv : the preprint server for biology · 2026Article
- Connections betweenInfection and immunity · 2026Article
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- Transmembrane Transcription Regulators Are Widespread in Bacteria and Archaea.Microbiology spectrum · 2023Article
- Structural Insights into Regulation of Vibrio Virulence Gene Networks.Advances in experimental medicine and biology · 2023Article
- CsrA Regulates Swarming Motility and Carbohydrate and Amino Acid Metabolism inMicroorganisms · 2021Article
- Vibrio cholerae TolC Is Required for Expression of the ToxR Regulon.Infection and immunity · 2021Article
- The periplasmic domains of Vibriocholerae ToxR and ToxS are forming a strong heterodimeric complex independent on the redox state of ToxR cysteines.Molecular microbiology · 2021Article
- σMolecular microbiology · 2021Article
- A disulfide constrains the ToxR periplasmic domain structure, altering its interactions with ToxS and bile-salts.Scientific reports · 2020Article
- Vibrio cholerae OmpR Contributes to Virulence Repression and Fitness at Alkaline pH.Infection and immunity · 2020Article
- Vibrio cholerae OmpR Represses the ToxR Regulon in Response to Membrane Intercalating Agents That Are Prevalent in the Human Gastrointestinal Tract.Infection and immunity · 2020Article
- Outer Membrane Vesiculation Facilitates Surface Exchange and In Vivo Adaptation of Vibrio cholerae.Cell host & microbe · 2020Article
- Ambient pH Alters the Protein Content of Outer Membrane Vesicles, Driving Host Development in a Beneficial Symbiosis.Journal of bacteriology · 2019Article
- Characterization of the Vibrio cholerae Phage Shock Protein Response.Journal of bacteriology · 2019Article
- Vibrio cholerae CsrA Directly RegulatesmBio · 2019Article
- Indole Inhibits ToxR Regulon Expression inInfection and immunity · 2019Article
- Environmental role of pathogenic traits inJournal of bacteriology · 2018Article
- The Vibrio cholerae RND efflux systems impact virulence factor production and adaptive responses via periplasmic sensor proteins.PLoS pathogens · 2018Article
- Bile salts and alkaline pH reciprocally modulate the interaction between the periplasmic domains of Vibrio cholerae ToxR and ToxS.Molecular microbiology · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Vibrio cholerae responds to environmental changes by altering the protein composition of its outer membrane. In rich medium, V. cholerae expresses almost exclusively the outer membrane porin OmpU, whereas in minimal medium, OmpT is the dominant porin. The supplementation of a minimal medium with a mixture of asparagine, arginine, glutamic acid, and serine (NRES) promotes OmpU production and OmpT repression at levels similar to those seen with rich media. Here we show that the altered Omp profile is not due to an increase in the growth rate in the presence of supplemental amino acids but requires the addition of specific amino acids. The effects of the NRES mix on Omp production were mediated by ToxR, a known regulator of omp gene expression. No changes in the Omp profile were detected in a toxR mutant. Supplementation with the NRES mix resulted in significantly higher levels of ToxR, and the elevated ToxR levels were sufficient to cause a switch in Omp synthesis. The increase in the level of the ToxR protein correlated with an increase in toxR mRNA levels and was observed only when toxR was expressed from its native promoter. ToxS, which is required for ToxR activity, was necessary for NRES-mediated omp gene regulation but not for the increase in ToxR levels. The growth of V. cholerae in the presence of bile acids also resulted in Omp switching, and this required ToxR. However, unlike the NRES mix, bile acids did not increase either ToxR protein or toxR mRNA levels, suggesting a different mechanism of omp gene regulation by bile than that by amino acids.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.