ArticleMethods in molecular biology (Clifton, N.J.)2012
Assessment of glial function in the in vivo retina.
Article in Methods in molecular biology (Clifton, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 12 citations in OpenAlex.
- Article
- Angiogenic neovessels promote tissue hypoxia.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Glial Cell Calcium Signaling Mediates Capillary Regulation of Blood Flow in the Retina.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Astrocyte structural reactivity and plasticity in models of retinal detachment.Experimental eye research · 2016Review
- Regulation of blood flow in the retinal trilaminar vascular network.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Purinergic control of vascular tone in the retina.The Journal of physiology · 2014Article
- Confocal microscopy for astrocyte in vivo imaging: Recycle and reuse in microscopy.Frontiers in cellular neuroscience · 2013Article
- A micro-advancer device for vitreal injection and retinal recording and stimulation.Experimental eye research · 2011Article
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Glial cells, traditionally viewed as passive elements in the CNS, are now known to have many essential functions. Many of these functions have been revealed by work on retinal glial cells. This work has been conducted almost exclusively on ex vivo preparations and it is essential that retinal glial cell functions be characterized in vivo as well. To this end, we describe an in vivo rat preparation to assess the functions of retinal glial cells. The retina of anesthetized, paralyzed rats is viewed with confocal microscopy and laser speckle flowmetry to monitor glial cell responses and retinal blood flow. Retinal glial cells are labeled with the Ca(2+) indicator dye Oregon Green 488 BAPTA-1 and the caged Ca(2+) compound NP-EGTA by injection of the compounds into the vitreous humor. Glial cells are stimulated by photolysis of caged Ca(2+) and the activation state of the cells assessed by monitoring Ca(2+) indicator dye fluorescence. We find that, as in the ex vivo retina, retinal glial cells in vivo generate both spontaneous and evoked intercellular Ca(2+) waves. We also find that stimulation of glial cells leads to the dilation of neighboring retinal arterioles, supporting the hypothesis that glial cells regulate blood flow in the retina. This in vivo preparation holds great promise for assessing glial cell function in the healthy and pathological retina.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.