ArticleMethods in molecular biology (Clifton, N.J.)2012
High-throughput flow cytometry bead-based multiplex assay for identification of Rho GTPase inhibitors.
Article in Methods in molecular biology (Clifton, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- A Novel Pharmacologic Activity of Ketorolac for Therapeutic Benefit in Ovarian Cancer Patients.Clinical cancer research : an official journal of the American Association for Cancer Research · 2015Trial
- Druggable targets in the Rho pathway and their promise for therapeutic control of blood pressure.Pharmacology & therapeutics · 2019Review
- Small-Volume Flow Cytometry-Based Multiplex Analysis of the Activity of Small GTPases.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Flow Cytometry: Impact on Early Drug Discovery.Journal of biomolecular screening · 2015Review
- Genetic barcoding with fluorescent proteins for multiplexed applications.Journal of visualized experiments : JoVE · 2015Article
- Quantitative bead-based flow cytometry for assaying Rab7 GTPase interaction with the Rab-interacting lysosomal protein (RILP) effector protein.Methods in molecular biology (Clifton, N.J.) · 2015Article
- A Pan-GTPase Inhibitor as a Molecular Probe.PloS one · 2015Article
- Article
- A Single-Cell Platform for Monitoring Viral Proteolytic Cleavage in Different Cellular Compartments.Biochemistry insights · 2015Article
- Targeting Rho-GTPases in immune cell migration and inflammation.British journal of pharmacology · 2014Review
- The intersection of flow cytometry with microfluidics and microfabrication.Lab on a chip · 2014Review
- Steady-state acceptor fluorescence anisotropy imaging under evanescent excitation for visualisation of FRET at the plasma membrane.PloS one · 2014Article
- Targeting GTPases in Parkinson's disease: comparison to the historic path of kinase drug discovery and perspectives.Frontiers in molecular neuroscience · 2014Review
- Implications of Rho GTPase Signaling in Glioma Cell Invasion and Tumor Progression.Frontiers in oncology · 2013Review
- Characterization of a Cdc42 protein inhibitor and its use as a molecular probe.The Journal of biological chemistry · 2013Article
- Novel multiplexed assay for identifying SH2 domain antagonists of STAT family proteins.PloS one · 2013Article
- Drug Repurposing from an Academic Perspective.Drug discovery today. Therapeutic strategies · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Rho family GTPases and their effector proteins regulate a wide range of cell signaling pathways. In normal physiological conditions, their activity is tightly controlled and it is not surprising that their aberrant activation contributes to tumorigenesis or other diseases. For this reason, the identification of small, cell permeable molecules capable of inhibition of Rho GTPases can be extraordinarily useful, particularly if they are specific and act reversibly.Herein, we describe a flow cytometric assay, which allows us to measure the activity of six small GTPases simultaneously. GST-tagged small GTPases are bound to six glutathione bead sets each set having a different intensity of red fluorescence at a fixed wavelength. The coated bead sets were washed, combined, and dispensed into 384-well plates with test compounds, and fluorescent-GTP binding was used as the read-out.This multiplex bead-based assay was successfully used for to identify both general and selective inhibitors of Rho family GTPases.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.