Evidence map›Paper›PMID 22144240›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2012

Identification of novel long noncoding RNA transcripts in male germ cells.

Tin-Lap Lee, Amy Xiao, Owen M Rennert

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Oocyte specific lncRNA variantNon-coding RNA research · 2021
    Article
  5. Article
  6. Long non-coding RNAs (lncRNAs) in spermatogenesis and male infertility.Reproductive biology and endocrinology : RB&E · 2020
    Review
  7. Long noncoding RNAs: new insights in modulating mammalian spermatogenesis.Journal of animal science and biotechnology · 2020
    Review
  8. Article
  9. Review
  10. Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Review
  17. Article
  18. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Tin-Lap LeeSchool of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, China. leetl@mail.nih.gov
Amy Xiao
Owen M Rennert

Funding

Studies of Pediatrics patients with genetic and metabolic disordersZIAHD008822 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI RENNERT, OWEN · 2009 to 2015
$6.4M
Transcription regulation and functional studies of germ cell specific genesZIAHD008817 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI RENNERT, OWEN · 2009 to 2013
$2.4M
Intramural NIH HHS ZIA HD008817
6 · The paper itself

Abstract

Emerging evidence from these studies suggested that the male germ cell transcriptome is more complex than previously envisioned. In addition to protein-coding genes, the transcriptome also encodes a significant number of nonprotein-coding transcripts. These noncoding (nc) RNAs appear to be involved in a variety of cellular activities, ranging from simple housekeeping to complex regulatory functions. A class of ncRNAs known as long ncRNAs (lncRNAs) were recently shown to be expressed in a developmentally regulated manner during brain and embryonic stem cell development. This protocol aims to predict and identify potential lncRNA candidates using Serial Analysis of Gene Expression (SAGE) data. We also illustrate how to validate the potential lncRNAs by expression analyses using real-time PCR and Northern Blot. Potential lncRNA candidates in male germ cells are identified using our previously established male germ cell SAGE database (GermSAGE).

Indexed as

Transcription, GeneticAnimalsBlotting, NorthernComputational BiologyGene Expression ProfilingGene Expression Regulation, DevelopmentalGene LibraryMaleMiceOpen Reading FramesReal-Time Polymerase Chain ReactionReproducibility of ResultsRNA, UntranslatedSpermatogoniaRNA, Untranslated

Identifiers

PMID22144240
PMCPMC3652227

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.